摘要
目的：建立山葡萄籽提取物中总多酚含量测定的方法。方法：以没食子酸为对照品,采用Folin-Ciocalteus比色法最适宜条件对山葡萄籽提取物中总多酚含量进行测定。结果：没食子酸对照品在浓度为0.002-0.010 g·L^-1范围内,与吸光度成良好的线性关系（Y=86.243X＋0.007,r=0.9996）;精密度试验RSD为0.14%;稳定性实验RSD为1.77%;重复性实验RSD为1.44%;平均回收率为102.00%,RSD为1.37%。结论：该法灵敏度高,重复性好,简便快捷。
Objective To establish the determination method of total polyphenols in vitis amurensis seed extract. Methods Using gallic acid as reference substance, the content of total polyphenols in Vitis amurensis seed extract was determined by the optimum conditions of Folin-Ciocalteu chromatometry. Results There was a good linearity between gallic acid concentration and absorbance in the range 0. 002--0. 010 g·L^-1（Y=86. 243X＋0. 007, r= 0. 9996）; the precision RSD was 0. 14%; the stability RSD was 1.77%; the repeatability RSD was 1.44%; the average recovery rate was 102.00~ （RSD= 1.37%）. Conclusion This method is simple, fast and has high sensitivity and good reproducibility.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2009年第5期877-879,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200302-05)
关键词
山葡萄籽
总多酚
没食子酸
比色法
Vitis amurensis seeds
total polyphenols
gallic acid
chromatometry
作者简介
贾荣（1982-），女，山西省原平市人，在读医学硕士，主要从事临床药学的研究。
[通讯作者]倪海镜（Tel：0431—84995158，E-mail：nihaij9966@126．com）
