摘要
目的检测哺乳动物斯钙素1(STC1)mRNA和STC1蛋白在二乙基亚硝胺(DENA)诱导大鼠肝癌发生不同阶段的表达水平。方法选取80只SD大鼠,随机分为两组,实验组按DENA 70 mg/kg体重灌胃,诱导大鼠肝癌模型,对照组等量蒸馏水灌胃。采用组织病理学、RT-PCR和Western blotting技术分析大鼠肝组织中的STC1基因和蛋白表达水平。结果只有在DENA诱导的大鼠肝癌组织内才有STC1 mRNA表达和STC1分泌,并且与肝癌发生进程具有相关性。结论STC1基因表达和STC1分泌水平与肝细胞癌变具有相关性,可望成为用于肝癌诊断的分子标记物。
Objective In the present study, levels of STC1 mRNA and STC1 were detected at various stages in rat hepatocarcinogenesis induced by diethylnitrosamine （DENA）. Methods Eighty Sprague-Dawley rats were divided into experimental group and the control group. In the experimental rats, the hepatocarcinogenesis was induced with diethylnitrosamine （DENA） through iutragastric gavage at a dose of 70 mg/kg body weight, and the control rats were done with distilled water at the same dosage. The cellular changes in liver were determined with histopathological techniques. Both of STC1 mRNA and STC1 level in liver were detected with RT-PCR and Western blotting technique, respectively. Results The results showed that both of STC1 mRNA and STC1 were only detected in liver of rats exposured to DENA. Conclusion The data suggested that there was correlation between the expression of STC1 gene and hepatocarcinogenesis, and implied STC1 as potential novel prognostic marker for detecting human liver cancer.
出处
《解剖学报》
CAS
CSCD
北大核心
2009年第5期760-765,共6页
Acta Anatomica Sinica
基金
河南省重点科技攻关计划(072102310085)
河南省动物学重点学科资助项目(KSF030018)
作者简介
张惠（1984-），女（汉族），河南省新乡市人，硕士研究生。
通讯作者：李卫国，E-mail：liwg0618@henannu．edu．cn，Tel：（0373）3326878
