摘要
建立以高效液相色谱法测定人参花中绿原酸含量的方法.色谱柱为ODS-C18(250mm×4.6mm,5μm),流动相为乙腈-0.5%磷酸溶液(30∶70),流速为1.0mL/mi n,检测波长为326nm,柱温为25℃.绿原酸在0.4～4.0μg范围内与峰面积积分值呈良好的线性关系(R=0.9991);平均回收率为99.28%,RSD=1.46%(n=6).本法简便、准确、重现性好、可用于人参花茶的质量控制和评价.
To establish a high-performance liquid chromatography ginseng flower content of chlorogenic acid method. Chromatographic ODS-C 18（250mm × 4.6mm, 5μm）, Mobile phase of acetonitrile -0.5% phosphoric acid （30：70）, flow1.0mL/min, The detection wavelength was 326nm, The column temperature was 25 ℃. Chlorogenie acid in the range of 0.4 × 4.0 μ g integral value within the peak area showed good linear relationship The average recovery was 99.28%, RSD=1.46%（n=6）. The method is simple, accurate, reproducible, and can be used for quality control of ginseng tea and its evaluation.
出处
《邵阳学院学报（自然科学版）》
2009年第3期74-76,共3页
Journal of Shaoyang University：Natural Science Edition
作者简介
刘松长（1955-），男，湖南安化人，高级讲师，主要从事应用化学研究
