摘要
目的:观察三皮汤对溃疡性结肠炎(UC)小鼠的疗效及探讨其治疗机制。方法:40只BALB/C小鼠随机分成正常组、模型组、柳氮磺胺吡啶(SASP)组和三皮汤组,每组10只。予以恶唑酮建立模型后,三皮汤组和SASP组分别给予三皮汤和SASP ig,每天观察小鼠体重、大便、饮食等情况,10天后处死小鼠,称脾重,取其病变结肠观察组织学改变,并采用酶联免疫吸附法(ELISA)测定各组小鼠血清白细胞介素(IL)-2及IL-10的含量。结果:三皮汤组和SASP组小鼠症状较模型组有明显改善;三皮汤组和SASP组小鼠脾组织重量较模型组有所增加;三皮汤组IL-2含量高于模型组(P<0.01),IL-10含量低于模型组(P<0.01)。结论:三皮汤对UC有确切疗效,且能升高血清IL-2及降低IL-10的含量,调整细胞因子间的网络平衡,改善机体免疫功能,控制肠道炎症。
Objective：To investigate the therapeutic effect of Sanpi Decotion in ulcerative colitis（UC） mice and discuss its therapeutic mechanism. Methods： Forty BALB/C mice were divided randomly and equally into normal group, model group, sulfasalazine（SASP） group, and Sanpi Decotion group（ten mice in each group）. After set up the oxazolone colitis, SASP group and Sanpi Decotion group were treated with sulfasalazine and sanpi decotion respectively, to observe the body weight, stool, food and drink situation of the mice every day. Killed all the mice on the tenth day, separated the spleen and weighed it, got the colon and evaluated the histological changes of the damaged colonic tissues, and measured the IL-2 and IL-10 concentrations in serum by using the enzyme-linked immunosorbent assay. Results： The symptom of Sanpi Decotion group and SASP group mice had great amelioration compared to model group mice, and compared to model group, the weight of spleen in Sanpi Decotion group and SASP group was increased, the concentration of IL-2 in Sanpi Decotion group was higher than that in model group（ P 〈 0.01）, and the concentration of IL-10 was lower than that in model group （P 〈 0.01 ）. Conclusion： Sanpi Decotion is effective for treatment of UC and it can increase the concentration of IL-2 and decrease the IL-10 concentration, regulate the balance of the cytokines, improve the function of immunity, control the inflammation in the colon.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第9期67-70,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖南省卫生厅中医药科研基金(062005)
作者简介
【通讯作者】蒋荣鑫，Tel：13787412737；E-mail：jrongxin@126．com
