摘要
FM4-64,a kind of membrane-selective fluorescent dye,was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici.The effect of FM4-64 on hypha was observed under confocal microscope.The results indicated that the plasma membrane,speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 μmol/L.The optimal dye concentration was 6 μmol/L for the both pathogens.At the same concentration of FM4-64,the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times.The staining of plasma membrane and vesicles should be completed in 5 minutes,as other organelles can be stained after 10 minutes.
FM4-64, a kind of membrane-selective fluorescent dye, was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici. The effect of FM4-64 on hypha was observed under confo- cal microscope. The results indicated that the plasma membrane, speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 ixmol/L. The optimal dye concentration was 6 μmol/L for the both pathogens. At the same concentration of FM4-64, the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times. The staining of plasma membrane and vesicles should be completed in 5 minutes, as other organelles can be stained after 10 minutes.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第4期435-438,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30671390)
作者简介
通讯作者：刘西莉，教授，主要从事杀菌剂药理学及病原菌抗药性研究；Tel（Fax）：010-62731013。E-mail：seedling@cau．edu．ca。
