摘要
将已扩增出的鸡堆型艾美耳球虫特异性单抗轻链可变区基因进行纯化,并用纯化的基因片段与pMD-18T载体连接,将重组载体转化于感受态细胞JM109,筛选出阳性重组子,提取阳性重组子质粒并进行测序,得到特异性单抗轻链可变区的基因序列。为鸡堆型艾美耳球虫特异性单链抗体基因的构建奠定了基础。
To study the function of small molecule antibody, light chain variable genes which was amplified from speciesspecific monoclonal antibodies against E. acervulina was purified, and cloned into clone vector pMD18-T by gene recombination techniques. The recombinant plasmid was transformed into E. coli JM109 by screening. Several recombinants that had been in serted with the target fragments were obtained. The positive clones containing recombination were identified by the methods of the gene sequencing. The result demonstrated that light chain variable gene was 312 bp.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第8期57-60,共4页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古民族大学硕士科研启动金项目
关键词
鸡堆型艾美耳球虫
单抗
可变区基因
克隆
测序
E. acervulina
monoclonal antibodies
variable genes
cloning
nucleotide sequencing
作者简介
康桂英（1976-），女，内蒙占人，硕士，研究方向：动物寄生虫病学。
