摘要
为研究口服纳米氧化锌抗堆型艾美耳球虫(Eimeria acervulina)感染的效果,将饲养至14日龄的雏鸡随机分成12组,每组30羽,分成3个重复。分别为空白对照组;纳米氧化锌剂量筛选试验:10×10^(4)孢子化卵囊感染鸡,分别口服去离子水对照和5、10、20、30 mg纳米氧化锌5组;纳米氧化锌抗不同剂量球虫感染试验:口服20 mg纳米氧化锌,分别用5×10^(4)、2.5×10^(4)、1×10^(4)孢子化卵囊进行感染及对应口服去离子水对照6组。试验结果显示,口服纳米氧化锌都能明显减少由于球虫感染引起的增重损失,提高相对增重率,其中每羽口服20 mg的效果最好;能减少球虫感染引起的死亡,提高存活率;能降低卵囊排出量;能减轻十二指肠的病变损伤;抗球虫指数明显高于相应对照组,且对低剂量的1×10^(4)球虫感染具有明显的治疗作用。研究表明,口服纳米氧化锌对堆型艾美耳球虫感染具有一定的防治作用。
The present study was to determine the effect of ZnO nanoparticles(ZnO NPs)fed to chickens against E.acervulina infection.Fourteen-day-old chickens were randomly divided 12 groups(10 chickens/cage and 3 cages/group)which were the blank control group;the five groups for the dose screening test of ZnO NPs,treated with drenched deionized water,5,10,20,and 30 mg ZnO NPs,respectively,and challenged with 10×10^(4)E.acervulina sporulated oocysts for all of the five groups;and the six groups for the anti-infection test,treated with drenched deionized water or 20 mg ZnO NPs and challenged with 5×10^(4),2.5×10^(4),1×10^(4)E.acervulina sporulated oocysts,respectively.The results showed that oral administration of ZnO NPs to the chickens increased their weight gain significantly,and the dose of 20 mg produced the best effect in this regard.ZnO NPs reduced the mortality and raised the survival of the infected chickens,decreased their oocyst excretion,lowered the lesion score of their duodenum,and improved their anti-coccidiosis index.ZnO NPs also had an efficient therapeutic effect on the chickens challenged with a low dose of 1×10^(4)E.acervulina sporulated oocysts.To sum up,oral challenge with ZnO NPs had a preventive and therapeutic effect on E.acervulina.
作者
徐立新
陈秋阳
史嘉雯
孙晓婷
宋小凯
严若峰
李祥瑞
XU Lixin;CHEN Qiuyang;SHI Jiawen;SUN Xiaoting;SONG Xiaokai;YAN Ruofeng;LI Xiangrui(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)
出处
《畜牧与兽医》
北大核心
2021年第7期78-82,共5页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31672545,31972705)。
作者简介
第一作者:徐立新,男,硕士,讲师,研究方向:兽医寄生虫学,E-mial:xulixin@njau.edu.cn。
