摘要
目的：建立高效液相色谱电化学法（HPLC-ECD）测定人血清中的儿茶酚胺（NE、E、DA）及5-羟色胺（5-HT）含量的方法。方法：运用高效液相色谱分离及电化学检测器检测,氧化铝固相萃取法测定NE、E、DA,5-磺基水杨酸蛋白沉淀法测定5-HT。结果：NE、E、DA在1-10 ng/ml,5-HT在10-100 ng/ml范围内线性良好（n=5,r均＆gt;0.998）,样品加标回收率为88%-105%,方法最低检出限：NE、E、DA为0.1 ng/ml,5-HT为1 ng/ml。结论：高效液相色谱电化学法（HPLC-ECD）测定人血清中的NE、E、DA及5-HT含量,该方法灵敏度高,回收率和重现性良好,样本用量少,操作快速简便,结果准确可靠。
Objectives：To establish a method to detect catecholamines such as nore-pinephrine（NE）,epinephrine（E） and dopamine（DA）,and 5-hydroxy tryptamine（5-HT） in human serum by high-performance liquid chromatography-electrochemical detection（HPLC-ECD）.Methods：HPLC and electrochemical detector were used to assay catecholamines and 5-HT in this research.Solid-phase extraction of alumina was applied for determination of NE,E and DA in human serum were determined by,while sulfosalicyclic acid was used as protein precipitator for determination of 5-HT in human serum.Results：Result of NE,E and DA showed a good linear relationship at the range of 110 ng/ml,and result of 5-HT indicated a good linear relationship at the range of 10100 ng/ml（n=5,r〉0.998）.The average recovery ratio was 88%105%.The limit of detection for NE,E and DA was 0.1 ng/ml,while that of 5-HT was 1 ng/ml.Conclusion：HPLC-ECD is a stable,highly sensitive and reproduciable method to determine NE,E,DA and 5-HT in human serum.
出处
《中国卫生检验杂志》
CAS
2009年第8期1722-1724,共3页
Chinese Journal of Health Laboratory Technology
作者简介
李静娜（1965-），女，学士，主管技师，主要从事理化检验工作。
