摘要
目的:通过观察大高良姜与红豆蔻乙酸乙酯部位对胃溃疡寒证大鼠的进食量、毛色、粪便、精神状态等体征的作用;测定大鼠的胃液量、胃液总酸度、胃蛋白酶活性和尿液中17-OHCS、儿茶酚胺(CAs)类物质的含量,初步探究大高良姜与红豆蔻乙酸乙酯部位对胃溃疡寒证大鼠发挥温胃散寒的作用机制。方法:取SPF级SD大鼠80只,设置空白组、模型组、阳性对照组、大高良姜与红豆蔻乙酸乙酯高低剂量组。采用冰乙酸和知母药液灌胃进行造模,造模成功后,空白组、模型组灌服蒸馏水,阳性对照组分别灌服有效剂量附子理中丸与西咪替丁,其余各组分别灌服受试药物,测定各组胃内相关指标与尿液中相关指标含量。结果:红豆蔻与大高良姜乙酸乙酯高低剂量组胃液量、胃蛋白酶活性、显著降低(P<0.01或P<0.05);胃酸pH值、尿液中17-OHCS、DA、NE、EP含量显著升高(P<0.01或P<0.05)。结论:红豆蔻与大高良姜乙酸乙酯部位可通过降低胃液体积量、胃蛋白酶活性,升高胃酸pH值、尿液中17-OHCS、DA、NE、EP含量,发挥其温胃散寒的功效及胃溃疡保护作用。
Objective:Observing Galangae Rhizoma and Galangae fructus ethyl acetate extractive impact to SD Experiment rats’signs such as food intake,coat color,faeces,mental status.Measure the rats'the gastric juice volume of rats,total gastric acidity value,total pepsin activity and contents of 17-OHCS of urine,catecholamine.Exploring preliminarily Galangae Rhizoma and Galangae fructus ethyl acetate extractive fraction’s warming stomach for dispelling cold mechanism of action which act on gastric ulcer and cold syndrome rats.Methods:Setup blank groups,model groups,positive control groups and the higher dose groups and the lower dose groups of Galangae Rhizoma and Galangae fructus ethyl acetate extractive fraction.The rat models were established by gavaging glacial acetic acid and Zhimu(Anemarrhena asphodeloides Bunge)water extract,after established successfully,blank group and model group gavage distilled water;positive control groups gavage aconitum lizhong pill and cimetidine in effective dose respectively,other groups gavage corresponding testmedication,measure the above-mentioned indexes in rats’stomach and urine of all groups.Results:In the higher dose groups and the lower dose groups of test-medication indexes:the gastric acid secretion,the total pepsin activity,the content of dopamine in the urine have statistically significant decrease(P<0.01 or P<0.05);the gastric pH,the content of 17-OHCS、DA、NE、EP in the urine have statistically significant increase(P<0.01 or P<0.05).Conclusion:Galangae Rhizoma and Galangae fructus ethyl acetate extractive fraction can work through decreasing the gastric acid secretion,the total pepsin activity,the content of dopamine in the urine and increasing the gastric pH,the content of 17-OHCS、DA、NE、EP in the urine to paly a role in warming stomach for dispelling cold and gastric ulcer resistance.
作者
杨凌霄
韦露玲
谢凤凤
李明芳
邓玲玉
易文燕
Yang Lingxiao;Wei Luling;Xie Fengfeng;Li Mingfang;Deng Lingyu;Yi Wenyan(The people’s hospital of HeZhou,HeZhou 542800;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Nanning 530200;Guangxi university of Chinese medicine,Nanning 530200)
出处
《壮瑶药研究》
2023年第1期161-165,314-315,共7页
Research of Zhuang and Yao Ethnic Medicine
基金
广西壮瑶药重点实验室(桂科基字[2014]32号)
壮瑶药协同创新中心(桂教科研[2013]20号)
广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函[2020]2605号)
关键词
大高良姜
红豆蔻
胃溃疡寒证
温胃散寒
Galangae Rhizoma
Galangae fructus
gastric ulcer cold syndrome
warming stomach for dispelling cold
作者简介
第一作者:杨凌霄,初级药师,主要从事医院药学研究;通讯作者:易文燕,副主任药师,主要从事医院药学、临床药学研究
