摘要
目的观察白芷香豆素(Coumarin of Angelicae Dahuricae,CAD)的镇痛作用部位及其机制。方法利用小鼠福尔马林实验及侧脑室注射CAD对小鼠热板痛反应潜伏期的影响以分析CAD的镇痛作用部位;观察CAD对甲醛所致伤害性疼痛模型小鼠血清一氧化氮(NO)和脑组织中β-内啡肽(β-EP)含量的影响。结果CAD(30,60,120 mg/kg)不同程度地抑制了小鼠福尔马林实验第一和第二时相反应。侧脑室注射CAD(6 mg/kg)明显延长小鼠热板痛反应潜伏期。CAD(30,60,120 mg/kg)连续给药4 d,使甲醛所致伤害性疼痛模型小鼠血清NO含量和脑中β-EP含量明显下降。结论CAD对物理、化学等伤害性刺激具有明显镇痛作用,作用部位可能既在中枢也在外周,镇痛机制可能与抑制NO的合成或释放有关。
Objective To observe analgesic site and mechanisms underlying of Coumarins of Angdicae Dahuricae （CAD）. Methods The anti-nocieeptive site of CAD was analysed with mice formalin test and mice hotplate test after intraeerebroventricular （icv） administration;the concentration of nitric oxide （NO） in serum and β-EP in brain of mice were measured with nitric reductase method and radioimmunoassay （RIA） respectively. Results CAD （30,60,120 mg/kg） reduced the firstand second-phase responses of mice formalin test compared with control group. After one time icy administration of CAD （6 mg/kg） for mice, hotplate latency were significantly prolonged. The concentration of NO in serum and β-EP in brain of mice induced by formalin were both decreased. Conclusions CAD could induce definite analgesic effect, which may be related to central and peripheral mechanisms.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第15期1902-1904,共3页
Chinese Journal of Gerontology
基金
吉林省科技厅重点资助项目(No.20050913)
作者简介
王海莉（1975-），女，医师，硕士，主要研究消化道肿瘤诊治。
通讯作者：崔新颖（1953-），女，教授，硕士生导师，主要研究神经药理学及心血管药理学。
