摘要
目的 :探讨中药蜈蚣对心肌缺血性损伤小鼠一氧化氮 (NO)及一氧化氮合酶 (i NOS)的影响。方法 :采用脑垂体后叶素造成小鼠心肌缺血性损伤模型。将健康小鼠分为四组 ,分别是空白组、模型组、蜈蚣小剂量组(2 .5 g/ kg)、蜈蚣大剂量组 (5 .0 g/ kg)。缺血 2 0 m in后取血观察乳酸脱氢酶 (L DH ) ,检测心肌组织一氧化氮 (NO) ,免疫组化法检测心肌一氧化氮合酶 (i NOS)。结果 :蜈蚣治疗后 L DH降低 ,NO、i NOS明显升高。结论 :蜈蚣可显著提高 NO及 i NOS的表达 ,这可能与蜈蚣保护血管内皮细胞功能有关。
Objective:To explore the effects of centipede on nitric oxide (NO) and iNOS in mice after myocardial injury.Method:Myocardial ischemia models were established by pituitrin.Micewere randomly divided into four groups,the control group,model group,small dose centipede group(2.5 g/kg)and big dose centipede group(5 g/kg).Every group had ten mice.After 20 minutes,myocardial ischemia mice's LDH and NO were observed.iNOS was determined by immunohistochemical assay.Results:After centipede treatment,LDH decreased.NO and iNOS expression increased.Conclusion:Centipede could significantly increased NO and iNOS expression.It may be related to centipede protect vascular endothelial cells.
出处
《山东中医杂志》
北大核心
2004年第8期492-494,共3页
Shandong Journal of Traditional Chinese Medicine
基金
河北省自然基金委员会资助项目 ( NO.3 972 74)