摘要
目的:对当归芍药散组方药味间配伍及剂量配比的变化对其治疗痛经效应的影响进行分析。方法:建立小鼠原发性痛经模型,采用正交t值法进行药味配伍分析,采用均匀设计法研究药味间剂量配比关系,以痛经小鼠扭体反应潜伏期及次数为主要评价指标,观察当归芍药散及其不同药味组合治疗痛经的作用。结果:正交t值法实验结果表明,芍药能够显著延长扭体潜伏期及减少扭体次数(P<0.01,P<0.05);当归及川芎能够显著延长扭体潜伏期(P<0.05)。茯苓、泽泻、白术对扭体潜伏期及次数影响均不显著。均匀设计结果表明,回归方程能够很好拟合各指标,各变量均有轻度曲面效应,通过曲面分析预测最佳配比为当归8.28∶芍药13.3∶川芎4.6,并对此进行了验证。结论:芍药对痛经小鼠扭体影响最为明显,当归、川芎次之,而茯苓、泽泻、白术起辅助作用。本研究为原方重用芍药提供了依据,并推测通过改变药味间配比可能产生更佳疗效。正交t值法及均匀设计等方法简便有效,能够适应中药方剂量效关系研究的需要。
AIM： To analyze the influence of different combinations and proportions of Danggui-Shaoyao-San＇s ingredients in treating primary dysmenorrhea. METHODS： The dysmenorrhea mouse model was established by oxytoein. The ingredient compati-bility was analyzed by orthogonal t design and the appropriate proportion relationship was researched by uni-form design. The latent period and number of twisting body were recorded to evaluate the intensity of dysmenorrhea. The algomenorrhea effect was observed with the treatment of Danggui-Shaoyao-San or the combination of different ingredients in a prescription. RESULTS： The orthogonal t design experimental results showed the latent period was significantly prolonged by Paeonia lactiflora Pall. （B）, Angelica sinensis Diels. （A） and Ligusticum chuanxiong Hort. （C）, the number of twisting body was decreased by B. Poria cocos Wolf. （ D ） , Alisma orientalis Juzep （E） and Atractylodes macrocephala Koidz. （F） had no significant influence on the latent period and the number of twisting body. The uniform design results showed the indexes were fitted with regression equation, the data of each variable had mild surface effect, the proper proportion between A： B ： C was 8.28 ： 13.3 ： 4.6 through surface analysis, and the above conclusion was proved. CON-CLUSION： The influnce on twisting body in algomenorrhea mouse of B was obvious, while A, C take the second place, D E, F played auxiliary function. The study gave strong support to the original formula to put B in a very important position, and it was speculated that slightly modulating the proportion of ingredients might bring better effects. The orthogonal t design and uniform design are valuable methods in studying multiingredient formulae of traditional Chinese medicine.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2009年第5期557-563,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
江苏省高校自然科学重大基础研究项目(06KJA36066)
江苏省方剂研究重点实验室开放课题(022021014002)资助
关键词
当归芍药散
痛经
量效关系
正交T值法
均匀设计
Danggui-Shaoyao-San
dysmenorrhea
dose-effect relationship
orthogonal t design
uniform design
作者简介
华永庆，男，博士，助理研究员，研究方向：中药药理。Tel：025—86798184 E-mail：huayongqing@126．com
段金廒，通信作者，男，博士，教授，博导，研究方向：方剂物质基础及中药资源化学研究。Tel：025-85811116 E-mail：dja@njutcm．edu.cn
