摘要
目的:探讨细胞周期和凋亡相关蛋白bcl-2和bax与脑胶质瘤病理分级的相关性,为脑胶质瘤严重程度的临床评价和机制研究提供参考依据。方法:取99例脑胶质瘤组织,分离获得单个胶质瘤细胞,采用FCM进行细胞周期和bcl-2及bax的测定,同时收集相应的病理分级资料,进行相关性分析和多元逐步回归分析。结果:病理分级与细胞周期(S期)及bcl-2、bcl-2/bax均呈正相关性,随着脑胶质瘤病理分级程度的升高,S期比例上升(t=2.48,P=0.018 4,R2=0.90),bcl-2、bcl-2/bax比值均上升(t=23.26,P<0.000 1,R2=0.93;t=27.42,P<0.000 1,R2=0.85)。结论:细胞周期S期分析和bcl-2、bcl-2/bax在脑胶质瘤严重程度的临床评价和机制研究中具有一定的参考价值。
Objective：To study the relationship between cell cycle, bcl-2 and bax expressions and the pathological grades of glioma in order to provide help for clinical evaluation and mechanism research of the severity of human glioma. Methods：This study collected 99 human glioma samples and isolated single glioma cells. Flow cytometry was used to detect their cell cycle and determine the expression levels of bcl-2 and bax. The pathological grading data were collected concurrently. The correlation and multiple stepwise re- gression analysis were analyzed by SAS software. Results：Pathologic grades had positive correlation with the percentage of glioma cells in S phase, the level of bcl-2, and the value of bcl-2/bax. As the increase in the pathologic grade, the percentage of glioma cells in S phase was also increased （t = 2.48, P=0.018 4, R2 = 0.90）, the expression level of bcl-2 and the ratio of bcl-2/bax were elevated （t = 23.26, P 〈 0.000 1, R2 = 0.93 ;t = 27.42, P 〈 0. 000 1, R2 = 0.85 ）. Conclusion： The percentage of glioma cells in S phase, the level of bcl-2, and the ratio of bcl-2/bax had some value for clinical evaluation and mechanism research of severity of glioma.
出处
《肿瘤》
CAS
CSCD
北大核心
2009年第6期578-581,共4页
Tumor
作者简介
Correspondence to：HUA Yong（华咏） E-mail ： jessicca-seh@ tom. com
