摘要
目的了解胡桃醌对人肝癌BEL-7402细胞亚显微结构的影响。方法12.5μmol/L胡桃醌作用于BEL-7402细胞24h后固定,分别行HE染色、考马斯亮蓝染色、扫描电镜及透射电镜样品制备。结果12.5μmol/L胡桃醌处理组细胞形态和细胞骨架发生改变。扫描电镜结果显示BEL-7402细胞胞体体积缩小,与周围细胞群脱离,表面微绒毛卷曲、畸变、小球结构逐渐增多,细胞间连接逐渐断裂,细胞间隙增宽,有凋亡小体形成。透射电镜结果显示胡桃醌作用BEL-7402细胞24h后,细胞内质网扩大,线粒体结构疏松,胞核核仁裂解,凋亡小体形成。结论胡桃醌能有效改变人肝癌BEL-7402细胞亚显微结构,诱导细胞凋亡。
Objective To study the effect of juglone on the ultrastructure of human liver cancer BEL-7402 cells. Methods BEL-7402 cells were incubated in the presence of 12.5 μmol/L juglone for 24 h, and fixed in 2.5% glutaraldehyde for HE staining and Coomassie brilliant blue staining and scanning electron microscopy. Results Incubation with juglone resulted in obvious changes in the cell morphology and cytoskeletal alterations of the cells. Scanning electron microscopy revealed reduced volume of the cell bodies, dissociation of the cells, curling and malformation of the microvilli on the cell surface with rupture of the intercellular junction and enlargement of the intercellular space. The formation of apoptotic bodies was observed. Transmission electron microscopy showed expansion of the endoplasmic reticula, mitochondrial cristea disintegration, nucleolar fragmentation and formation of the apoptotic bodies after the exposure to juglone for 24 h. Conclusion Juglone can cause ultrastructural changes of human liver cancer BEL-7402 cells and induce their apoptosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第6期1208-1211,共4页
Journal of Southern Medical University
作者简介
陈丽，（1981-），女，博士研究生，电话：020-62789158，E-mail：yaligj@126．com
通讯作者：顾为望（1956-），男，教授，研究方向：比较医学，实验动物新品种培育
