摘要
AIM： To investigate the effects of tachyplesin and n-sodium butyrate on proliferation and gene expression of human gastric adenocarcinoma cell line BGC-823. METHODS： Effects of tachyplesin and n-sodium butyrate on proliferation of BGC-823 cells were determined with trypan blue dye exclusion test and HE staining. Effects of tachyplesin and n-sodium butyrate on cell cycle were detected by flow cytometry. Protein levels of c-erbB-2, c-myc, p53 and p16 were examined by immunocytochemistry. RESULTS： The inhibiting effects were similar after 2.0 mg/L tachyplesin and 2.0 mmol/L n-sodium butyrate treatment, the inhibitory rate of cellular growth was 62.66% and 60.19% respectively, and the respective maximum mitotic index was decreased by 49.35% and 51.69% respectively. Tachyplesin and n-sodium buD/rate treatment could markedly increase the proportion of cells at G0/G1 phase and decrease the proportion at S phase. The expression levels of oncogene c-erbB-2, c-myc, and mtp53 proteins were down-regulated while the expression level of tumor suppressor gene p16 protein was up-regulated after the treatment with tachyplesin or n-sodium buD/rate. The effects of 1.0 mg/L tachyplesin in combination with 1.0 mmol/L n-sodium butyrate were obviously superior to their individual treatment in changing cell cycle distribution and expression of c-erbB-2, c-myc, mtp53 and p16 protein. The inhibitory rate of cellular growth of BGC-823 cells after combination treatment was 62.29% and the maximum mitotic index wasdecreased by 51.95%. CONCLUSION： Tachyplesin as a differentiation inducer of tumor cells has similar effects as n-sodium butyrate on proliferation of tumor cells, expression of correlative oncogene and tumor suppressor gene. It also has a synergistic effect on differentiation of tumor cells.
瞄准：在增长和人的胃的腺癌房间线 BGC-823 的基因表示上调查 tachyplesin 和 n 钠丁酸盐的效果。方法：BGC-823 房间的增长上的 tachyplesin 和 n 钠丁酸盐的效果与尝试平底锅蓝色染料排除测试被决定并且他染色。房间周期上的 tachyplesin 和 n 钠丁酸盐的效果被流动血细胞计数检测。c-erbB-2， c-myc， p53 和 p16 的蛋白质层次被免疫细胞化学检验。结果：禁止的效果在 2.0 mg/L tachyplesin 和 2.0 mmol/L n 钠丁酸盐处理以后是类似的，细胞的生长的禁止的率分别地是 62.66% 和 60.19% ，并且各自的最大的有丝分裂的索引被 49.35% 和 51.69% 分别地减少。Tachyplesin 和 n 钠丁酸盐处理能显著地在 /G (1 ) 分阶段执行并且减少的 G (0 ) 增加房间的比例在 S 的比例阶段。当表示肿瘤铺平时， oncogene c-erbB-2， c-myc，和 mtp53 蛋白质的表示层次是下面调整的压制或基因 p16 蛋白质在有 tachyplesin 或 n 钠丁酸盐的处理以后是起来调整的。在有 1.0 mmol/L n 钠丁酸盐的联合的 1.0 mg/L tachyplesin 的效果比他们在改变房间周期分发和 c-erbB-2， c-myc， mtp53 和 p16 蛋白质的表示的单个处理显然优异。在联合处理以后的 BGC-823 房间的细胞的生长的禁止的率是 62.29% ，最大的有丝分裂的索引被 51.95% 减少。结论：肿瘤房间的区别 inducer 在肿瘤房间， oncogene 和肿瘤压制的关联词的表示或基因的增长上作为 n 钠丁酸盐有类似的效果的 Tachyplesin。它也在肿瘤房间的区别上有协同的效果。
基金
Supported by the National Natural Science Foundation of China, No.30170724
作者简介
Correspondence to： Dr. Qi-Fu Li, The Key Laboratory of Chinese Ministry of Education for Cell Biology ＆ Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen 361005, Fujian Province, China. chifulee@ 163.net Telephone： ＋86-592-2183619 Fax： ＋86-592-2186392
