摘要
实时定量PCR技术现已广泛应用于细胞或组织mRNA转录水平的检测和半定量。选择合适的内参基因可以消除不同标本在RNA的产量、质量以及逆转录效率上可能存在的差别,从而获得目标基因特异性表达的真正差异。本试验应用实时定量PCR技术,研究小鼠在经过免疫刺激后,B2M、ACTB、GAPDH、SDHA、HPRT1和ARBP共6个内参基因在肝脏中的表达情况。结果表明,这6个内参基因表达存在差异。经过geNorm程序统计学分析,确定了ACTB、GAPDH两个看家基因适用于校正目标基因的表达量,为研究小鼠免疫刺激后肝脏目标基因的表达奠定基础。
Real-time quantitative PCR is the technique of choice for detection and quantification of mRNA expression in cells or tissues. Suitable reference gene is essential for high precision of target gene by taking the RNA quality and efficiencie of reverse transcription into account. To investigate the expression of B2M, A CTB, GAPDH, SDHA, HPRT1 and ARBP in mouse liver after immunity treatment by RT-PCR. Differences in expression levels were observed by geNorm analysis. A CTB and GAPDH were finally determined as suitable housekeeping genes. It is meaningful for research of target gene in mouse liver after immunity treatment.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2009年第5期80-85,共6页
Journal of Northeast Agricultural University
基金
国家自然基金项目(30871837)
动物营养学国家重点实验室自主研究课题(2004DA125184(青)0801)
作者简介
董晓丽（1983-），女，山东人，硕士研究生，研究方向为反刍动物营养。
通讯作者：研究员，博士生导师，Email：wang-jia-qi@263．net
