摘要
目的:对比苦石莲皮和仁中挥发性成分。方法:利用有机溶剂-水蒸气蒸馏法提取苦石莲挥发油,用GC-MS进行测定,色谱柱为HP-5MS5%Phenyl Methyl Siloxane弹性石英毛细管柱(30m×0.25mm×0.25μm),结合计算机检索技术对分离的化合物进行结构鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果:苦石莲皮中分离出115个化学成分,鉴定出63个化学成分,占总挥发性成分的54.7%,其中相对百分含量大于2.0%有5种;苦石莲仁中分离出102个化学成分,鉴定出51个化学成分,占总挥发性成分的50.0%,其中相对百分含量大于2.0%有7种。苦石莲皮和仁挥发性成分中有40种相同成分,其中皮中含量最高的是柠檬烯(5.31%),仁中含量最高的是己醇(12.94%)。结论:本实验首次对苦石莲皮和仁挥发性成分进行对比研究。为有效控制苦石莲药材质量及深度开发该药材提供参考数据。
Objective： To compare the volatile chemical constituents from the shell and kernel of Caesalpinia minax Hance. Methods： The volatile chemical compositions of Caesalpinia minax Hance were obtained by organic solventwet distillation and were analyzed by GC-MS equipped with a elastic quartz capillary column HP-5MS 5% Phenyl Methyl Siloxane （30m×0.25mm×0.25μm）. The constituents were identified by their mass spectra. The relative percentage of the volatile constituents was calculated from the GC peak areas. Results： One hundred and fifteen kinds of chemical constituents in the shell of Caesalpinia minax Hance were separated and sixty-three of them, which accounts for 54.7% of total volatile constituents, were characterized. There are five kinds whose relative contents were more than 2.0%. One hundred and two kinds of chemical constituents in the kernel of Caesalpinia minax Hance were separated and fifty-one of them, which accounts for 50.0%, were characterized. There are seven kinds whose relative contents were more than 2.0%. Form the shell and kernel of Caesalpinia minax Hance there were forty kinds of chemical constituents the same. The most relative contents were Limonene （5.31%, shell） and 1-Hexanol （12.94%, kernel）. Conclusion： This paper reports, for the first time, the comparison of volatile constituents from shell and kernel of Caesalpinia rninax Hance.It is helpful for controlling the quality and further researching of Caesalpinia minax Hance.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2009年第6期783-786,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
贵州中药材挥发性成分的研究(No.黔省专合字[2005]75号)
作者简介
通讯作者：袁月玲，贵州省贵阳市解放西路南冲巷17号，邮编：550002，电话：0851-5713626，传真：0851-5792049 E-mail：yuanyueling@yahoo．com．cn
