摘要
目的:探讨球花石斛Dendrobium thyrsiflorum不同生长发育时期叶和根中香豆素类(coum arins)成分的分布与动态变化,为充分利用药用石斛资源提供实验依据。方法:采集球花石斛营养生长期和生殖生长期的叶、根,利用激光共聚焦显微技术观察、分析其所含香豆素类成分。结果:球花石斛的叶除叶脉近上、下表皮处的维管束纤维群外,其他部位均有香豆素类成分的存在;球花石斛根的各部位均有香豆素类成分存在,尤其在皮层以内细胞的壁和壁内周。球花石斛叶、根的香豆素类成分的相对含量在生殖生长中的盛花期均高于营养生长期;在生殖生长进入始果期二者均几无香豆素类成分分布。结论:为充分利用药用植物,可从球花石斛的叶、根中提取香豆素类成分。
Objective ： To study the distribution and dynamic change of coumarins in the leaf and root of Dendrobium thyrsiflorum at different developmental stages, and provide an experimental evidence for utilizing the medicinal plant. Method： The leaf and root materials were collected at vegetative and reproductive stage. By applying laser scanning confocal microscopy, relative quantity of coumarins of each sample was observed and analyzed. Result： Coumarins located in D. thyrsiflorum leaf except fibers in vascular bundles which were close to the upper or lower epidermis. Coumarins were found in D. thyrsiflorum root structure at different developmental stages and walls of cells within cortex were especial appropriate places for the components distribution. Relative quantity of coumarins was changed obviously when plant was growing. The components reached the highest level during profuse flowering period whereas there were less coumarins at vegetative period and little during initial fruit period. Conclusion： In order to utilize D. thyrsiflorum reasonably and thoroughly, coumarins should be extracted from the plant leaf and root.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第9期1071-1074,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30171144)
安徽省高校生物环境与生态安全省级重点实验室专项项目
关键词
球花石斛
叶
根
香豆素
分布
动态变化
激光共聚焦显微技术
Dendrobium thyrsiflorum
leaf
root
coumarin
distribution
dynamic change
LSCM （laser scanning confocal microscopy）
作者简介
[通信作者]王峥涛，Tel：（021）51322519，E-mail：wangzht@hotmail．com
