摘要
采用RT-PCR技术克隆了甘薯肌醇-1-磷酸合酶基因的cDNA编码区,命名为IbMIPS-1。该基因由1530个碱基组成,编码510个氨基酸,与蓖麻、烟草、芝麻、大豆肌醇-1-磷酸合酶的氨基酸序列比对表明,其同源性很高。将克隆的IbMIPS-1基因连接到pQE30载体,构建原核表达载体。实时定量RT-PCR分析结果表明,在茎线虫侵染甘薯块根后,IbMIPS-1基因被诱导表达,并且在侵染后的第4天表达量最高。推测该基因可能参与甘薯抗茎线虫病信号传导,以诱发甘薯茎线虫病抗性反应。
The cDNA of myo inositol- 1-phosphate synthase （MIPS） gene was successfully cloned by using real-time quantitative RT-PCR in sweetpotato. This gene contained 1 530 bp and encoded 510 amino acids, named IbMIPS-1. Sequence analysis indicated that IbMIPS-I had high homology with Ricinus communis MIPS gene, Nico- tiana tabacum MIPS gene, Sesamum indicum MIPS gene and Glycine max MIPS gene. The cloned cDNA of Ib- MIPS-1 gene was introduced into pQE30 vector, giving a prokaryotic expression vector. Results of real-time quantitative RT-PCR analysis showed that the expression of IbMIPS-1 gene was induced by sweetpotato stem nema- todes and the highest expression level was observed at the fourth day after infected by sweetpotato stem nematodes. The results provide evidence that IbMIPS-1 gene may play an important role in primary response to the infected stem nematodes in sweetpotato.
出处
《分子植物育种》
CAS
CSCD
2009年第3期537-544,共8页
Molecular Plant Breeding
基金
国家自然科学基金(30871570)
国家科技支撑计划(2006BAD01A06)
农业部948项目(2006-G21-01)共同资助
关键词
甘薯
IbMIPS-1基因
茎线虫
原核表达载体
实时定量RT—PCR
Sweetpotato （lporrtoea batatas （L.） Lam.）, IbMIPS-1 gene, Stem nematode, Prokaryotic expression vector, Real-time quantitative RT-PCR
作者简介
通讯作者，liuqc@cau．edu．cn
