摘要
目的研究花锚提取物中3种酮苷对肝脏保护作用。方法用CC l4灌胃小鼠,建立急性肝损伤动物模型,观察其肝脏组织形态,测定肝脏系数,血清中谷丙转氨酶、谷草转氨酶活性和肝糖元含量,研究3种酮苷对小鼠肝损伤的保护作用。结果模型组中小鼠的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性、肝脏系数、肝糖元A值分别为(1045.61±47.82),(1323.52±230.22)U/L,(0.062±0.0041),(0.062±0.013);与模型组比较,花锚中3种酮苷高剂量组(10 mg/m l)对小鼠实验性肝损伤均有明显的修复作用,能降低CC l4所致肝损伤小鼠的肝脏系数、血清ALT、AST活性,并提高肝糖元含量(P<0.01);化合物A、B、C中剂量组(5 mg/m l)的肝糖元A值分别为(0.072±0.011),(0.077±0.015),(0.078±0.012),与模型组相比均明显升高(P<0.05),其余各项指标与模型组比较,差异均有统计学意义(P<0.01);与模型组比较,化合物B低剂量组(1 mg/m l)可有效降低小鼠的肝脏系数及血清AST活性(P<0.05),化合物C低剂量组可明显降低小鼠血清AST活性(P<0.01),对ALT活性的升高也有较好的抑制作用(P<0.05),而其他给药组与模型组比较,差异均无统计学意义。结论花锚中3种酮同苷均有较好的保肝作用,为花锚保护肝脏的有效成分、且具有一定的量效关系。
Objective To research the protective effect of three xanthone glucosides from Halenia corniculata on chemical hepatic injury in mice. Methods The chemical hepatic injury was induced by CCl4 in mice as the model to study the morphologic changes of liver and the liver coefficient, serum ALT, AST activity and liver glycogen level. Results Compared with the model group,the high dose（ 10 mg·ml^-1 ） xanthone glucosides showed effects of restoring of liver tissue,reducing the liver coefficient, serum ALT and AST activity, increasing the liver glycogen level（ P 〈 0. 01 ）. The medium dose （ 5 mg·ml） xanthone glucosides increased the liver glycogen level（ P 〈 0. 05 ） and induced significant alterations of other indexes compared with model group（P 〈0. 01）. In low dose （ 1 mg/ml） ,B type xanthone glucoside reduced the liver coefficient and serum AST activity （P 〈 0. 05 ）. The C type xanthone glucoside significantly reduced the serum AST activity（ P 〈 0.01 ） and inhibited the rise of the serum ALT（ P 〈 0. 05 ）. Conclusion The results indicate that three xanthone glucosides from Helenia corniculata could protect the liver against chemical injury in dose-effect manner in mice.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2009年第5期587-589,共3页
Chinese Journal of Public Health
作者简介
张进（1982-），女，辽宁庄河人，博士在读，研究方向：天然产物成分的提取分离及活性研究。
通讯作者：孙启时
