摘要
研究了嗜酸乳杆菌的高密度培养条件,并使用喷雾干燥法对嗜酸乳杆菌粉末发酵剂进行了制备。实验表明,嗜酸乳杆菌6012的最佳增菌培养基为:乳清培养基+06.%(w/v)低聚异麦芽糖+06.%(w/v)黄豆粉+10%(v/v)胡萝卜汁+05.%(w/v)CaCO3。在培养过程中使用20%柠檬酸钠调节培养基pH值为62.,37℃静置培养18 h,活菌数可达10.×109cfu/mL。以0.5%甘油+0.5%葡萄糖+3%大豆分离蛋白+2%海藻酸钠作为抗热保护剂,菌体经过喷雾干燥,其发酵剂活菌数为80.×108cfu/g。
The conditions of high density culture of Lactobacillus acidophilus and the production of starter powder were studied in this paper. The optimal culture conditions of L. acidophilus 6012 were obtained as followed ： whey medium, 0.6%（w/v） isomahooligosaccharide, 0.6%（w/v） soybean, 10% （v/v） carrot juice and 0.5% （w/v） CaCO3. pH was adjusted to 6.2 by addition of 20% citric acid sodium. The viable number could reach 8.9 ×10^8 cfu/mL after cultured for 18 h at 37℃. The optimal protective agents were 0.5% glycerol, 0.5% glucose, 3% isolate soy protein and 2% sodium alginate. Under these conditions, the viable number of L. aciclophilus in starter powder was 8.0 ×10^8 cfu/g after spray drying.
出处
《中国酿造》
CAS
北大核心
2009年第5期111-116,共6页
China Brewing
关键词
嗜酸乳杆菌
高密度培养
发酵剂
Lactobacillus acidophilus
high density culture
starter powder
作者简介
作者简介：王艳萍，女，天津人，教授，研究方向为食品微生物。
