摘要
为研制出一种能够同时选择性富集沙门氏菌、志贺氏菌和金黄色葡萄球菌的培养基,对多种抑制剂和促进剂进行筛选。实验优化出的最佳培养基配方为:胰蛋白胨15g、大豆蛋白胨5g、磷酸二氢钾2.5g、葡萄糖2.5g、氯化钠35g、氯化锂0.5g、牛胆盐0.1g、甘露醇2g、亚碲酸钾0.3mg,蒸馏水1000mL。沙门氏菌、志贺氏菌和金黄色葡萄球菌等目标细菌在此共增菌培养基中均能快速生长,以103CFU/mL为初始菌浓,18h后菌浓能达到107CFU/mL,而非目标菌在此肉汤中的生长受到抑制。结果表明,此培养基可以用于沙门氏菌、志贺氏菌和金黄色葡萄球菌的选择性共增菌。
In order to develop an enrichment broth for the simultaneous growth of Salmonella, Shigellosis and Staphylococcus, several candidate agents were added to pre-formulated broth to observe their growth speeds. The optimal performance was obtained with the enrichment broth composed of 15 g of tryptone, 5 g of soya peptone, 2.5 g of monopotassium phosphate, 2.5 g of glucose, 35 g of sodium chloride, 0.5 g of lithium chloride, 0.1 g of bile salt, 2 g of mannitol and 0.3 g of potassium tellurite in 1000 mL of distilled water. Results showed that Salmonella, Shigellosis and Staphylococcus aureus could concurrently enrich in this broth and the concentration of bacteria could increased from 103 CFU/mL to 107 CFU/mL during 18 h. The growth of non-target bacteria was suppressed in this broth. Therefore, it may be a promising broth for simultaneous detection of the three pathogens in a single platform.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第17期171-175,共5页
Food Science
基金
惠州市科技计划项目(0031939140712048)
广东省科技计划项目(2011B020314004)
作者简介
王永志（1988-），男，硕士研究生，主要从事食品安全检测方法研究。E—mail：1wangyongzhi@gmail．com
通信作者：肖性龙（1977-），男，助理研究员，博士，主要从事食品安全检测研究。E-mail：fexxl@scut．edu．cn
