摘要
以海藻酸钠为载体,研究了木聚糖酶的固定方法,并利用固定化酶和游离酶进行了酶解试验。结果表明:固定化酶的最适温度,热稳定性,最适pH,稳定性及与底物的亲和力都有所提高。其中采用直接包埋方式固定率低,稳定差;交联包埋法固定的固定化酶反应pH范围变宽,固定率高,但与底物的亲和能力强和操作稳定性不如包埋交联法。
Xylanase was immobilized by using sodium alginate as carrier. The immobilized methods were studid. The immobilized xylanase was used to hydrolyze xylan. The results showed that the optimum temperature,thermal stability, optimal pH and pH stability of ira-mobilized xylanase had increased. And its affinity for substrate was improved. Immobilized xylanase made by direct-embedded method had low immobilized rate and bad stability. The reaction pH of immobilized xylanase using cross-linking of embedded method broadens , and the immobilized rate was high. But its affinity for substrate and operation stability were behind embedded cross-linking method.
出处
《北方园艺》
CAS
北大核心
2009年第5期92-94,共3页
Northern Horticulture
关键词
木聚糖酶
海藻酸钠
固定化
Xylanase
Sodium alginate
Immobilization
作者简介
王娜（1986-），女，山东寿光人，本科在读，研究方向为生物科学。E-mail：changfengchiluoye@126．com。
通讯作者：朱启忠（1957-），男，山东单县人，教授，硕士研究生导师，现从事生物化学的教学和科研工作。E—mail：hzzqz@sdu．edu．cn。
