摘要
以蟹壳为原料提取壳聚糖，用戊二醛作交联剂，将纤维素酶固定于壳聚糖上．同时探讨了一定量干壳聚糖载体与交联剂浓度、给酶量等关系的最适固定化酶条件，并对固定化酶的热稳定性、操作稳定性、米氏常数、最适温度、离子强度的影响及使用半衰期等理化性质进行了探讨．
Chitosan was obtained from crab shells by treating with HCl and NaOH. The conditions of cellulase immobilized on chitosan by glutaraldehyde were studied. The results indicated that the immobilized-cellulase prepared by 0. 1g dry chitosan cross-linking with 5%glutaraldhyde and then combining with 4.0 mg cellulase showed higher cellulase activity and better activity recoveries (75% ).And some characters of immobilized and native cellulases, such as optimum temperature Michaelis constant and the effect of ionic strength, stabilities to heat and repeat operation were studied and compared.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1996年第3期250-254,共5页
Progress In Biochemistry and Biophysics
关键词
纤维素酶
壳聚糖
固定化
chitosan
cellulase
immobilization
glutaraldehyde
