摘要
以杂种白杨‘717杨’（Populus tremula×Populusalba）和‘353杨’（Populustremula×Populus tremuloides）的叶片为材料，研究不同基因型、激素组合对叶片分化和不定芽生根的影响。结果表明：‘717杨’叶片最适分化培养基是MS＋0．5mg／L6-BA＋0．2mg／LNAA，‘353杨’叶片最适分化培养基是MS＋0．5mg／L 6-BA＋0．2mg／LNAA＋0．01mg／LTDZ；最佳生根培养基为1／2MS＋0．2mg／LIBA＋0．2mg／LNAA，生根率达100％；叶片培养的最佳位置是自茎尖展叶的1～3片叶；20mg／L卡那霉素可以抑制‘717杨’和‘353杨’叶片的诱导分化，40mg／L卡那霉素可以抑制‘717杨’和‘353杨’不定芽的生根。
The regeneration of adventitious buds and the rooting of shoots from the leaves of hybrid 717 from Populus trernula × Populus alba and 353 from Populus trernula × Populus trernuloides with different genotypes and hormones combination were studied. The results showed that： the optimum medium for ad- ventitious buds regeneration of hybrid 717 was MS＋0.5 mg/L 6 BA＋0.2 mg/L NAA;The optimum medium for adventitious buds regeneration of hybrid 353 was MS＋0.5 mg/L 6-BA＋0.2 mg/L NAA＋0.01 mg/L TDZ;The optimum medium for rooting of shoots was 1/2 MS＋0.2 mg/L IBA＋0.2 mg/L NAA, with a rooting efficiency of 100%. The best leaves for adventitious buds regeneration were on the tip 1st～ 3rd leaf of stems. 20 mg/L kanamycim could inhibit the bud regeneration of the hybrid 717 and 353,40 mg/ L kanamycim could inhibit the root regeneration of the hybrid 717 and 353 from the shoots.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第4期704-710,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
教育部长江学者奖励计划
关键词
白杨
叶片
不定芽分化
再生体系
poplar
leaves
adventitious buds regeneration
regeneration system
作者简介
王斌（1982-），女（汉族），在读硕士研究生，主要从事杨树转基因研究。E—mail：wangbin10256@163．com
通讯作者：张金凤，博士，教授，硕士生导师，主要从事林木遗传育种研究。E-mail：zjf@bjfu．edu．cn
