摘要
本文建立了食品中碱性桃红T染料含量的高效液相色谱检测方法。样品经碱化甲醇提取,在碱性条件下用聚酰胺固相萃取小柱富集、净化,酸化甲醇洗脱后经高效液相色谱仪分离、检测。该方法的碱性桃红T检测限为0.004mg/kg。6种食品样品回收率为76.1%～96.5%,相对标准偏差(RSDs)为0.8%～7.6%(n=6),在0.1～5μg/mL浓度范围内呈良好的线性关系,线性回归系数r=0.9999。
A method for determination of Safranine T in food by high performance liquid chromatography （HPLC） has been developed. The food sample was extracted by basified methanol, cleaned up with polyamide SPE cartridge and determined by HPLC. The linear range for dye was 0.1 μg/mL- 5μg/mL; r=-0.9999. The recoveries were 76.1%- 96.5%, and the relative standard deviations were 0.8%-7.6%（n=6）.The detection limits were about 0.004mg/kg for Safranine T.
出处
《福建分析测试》
CAS
2009年第2期9-11,共3页
Fujian Analysis & Testing
关键词
高效液相色谱法
食品
碱性桃红T
High performance liquid chromatography
Food
Safranine T
作者简介
作者简介：何树坤（1963-），男，高级工程师，从事色谱分析检验工作。Email：fjqs@fcii．net
