摘要
提出了高效液相色谱法测定氧化型染发剂中11种染料含量的方法。样品用甲醇-水(1+1)溶液超声萃取15min,经Capcell Pak C18MG S5色谱柱(250mm×4.6mm,5μm)分离,用磷酸调至pH 7.7的乙腈-三乙胺磷酸缓冲溶液(3+97)为流动相进行等度洗脱,用二极管阵列检测器于波长280nm处检测。11种染料的线性范围均在20～500mg·L-1之间。以空白染发剂样品为基体,进行加标回收试验,回收率在70%～110%之间,相对标准偏差(n=6)均小于10%。
HPI.C was applied to the determination of 11 dyes in oxidative hair colorants. The sample was extracted with mixture of methanol and H2O （1＋1）. The extract was separated on Capcell Pak Cls MG S5 column （250 mm×4. 6 mm, 5 μm by gradient elution with mixture of acetonitrile and triethylamine （adjusted to pH 7. 7 with phosphoric acid） mixed in the ratio of 3 to 97, and determined with photodiode array detector at the wavelength of 280 nm. Linearity ranges for 11 dyes were found between 20-500 mg ·L-1. Using blank sample of hair colorants as matrix, test for recovery was made by standard addition method, values of recovery found were in the range of 70%-110% with RSD＇s （n=6） less than 10%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第11期1347-1349,1352,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
浙江省大型科学仪器设备协作共用平台科技计划项目(2009F70038)
作者简介
吕春华（1981-），女。浙江东阳人，博士，主要从事理化检验。E—mail：lvch@ziq．gov．cn
