摘要
目的探讨益气补肾口服液(YQBS)对体外肿瘤浸润淋巴细胞(TIL)的作用,并从免疫学角度初步探讨其作用机制。方法采用不连续密度梯度离心法分离T淋巴细胞,观察YQBS对体外TIL培养后存活时间的影响;TIL体外杀伤活性的影响;TIL细胞形态学的变化。结果YQBS明显延长TIL体外存活时间;对自体瘤细胞、K562细胞的杀伤活性均有显著增强;经细胞涂片和电镜观察,YQBS培养后1周左右,TIL明显增多。结论YQBS具有明显的增加体外TIL数量和活性的作用,其抗肿瘤的作用可能与提高机体免疫能力有关。
Objective： To study the effect of Yiqi Bushen oral liquid YQBS on tumor-infiltrating lymphocytes TIL in vitro and its related immunological Mechanism. Methods： Separation of T-lymphocyte by Discontinuous density gradient centrifugation Observe the impaction of YQBS on survival of TIL .Results： YQBS could prolong survival time of TIL significantly and Enhanced the Killing activity of Autologous on tumor cells and K562 cells Moreover, the cell smear and electron microscopy analysis showed that TIL growth increased significantly by culturing about one week. Conclusion： ~QBS could increase the growth and the activity of TIL Notably the mechanism of anti-tumor effects of YQBS might be related to the strengthened immunity function of mice.
出处
《当代医学》
2009年第4期136-138,共3页
Contemporary Medicine
关键词
益气补肾口服液
肿瘤
免疫
Yiqi Bushen oral liquid
tumor
immunity
