摘要
目的通过检测急性自发性脑出血患者白细胞介素-18(IL-18)和干扰素-γ(IFN-γ)的血清水平值,探讨IL-18、IFN-γ在急性自发性脑出血中的发病机制和作用,研究IL-18、IFN-γ与急性自发性脑出血急性发病后的氧化应激效应的关系。方法实验分为两组:正常组(N组)与脑出血组。采用酶联免疫吸附测定法(ELISA)检测急性自发性脑出血患者血清IL-18、IFN-γ浓度值,硫代巴比妥酸法测定MDA、乳过氧化物酶法测定血清LPO水平。结果IL-18与IFN-γ在急性自发性脑出血患者中明显高于对照组,差异有统计学意义(P<0.05)。且患者血浆IL-18与IFN-γ呈显著正相关(P<0.05);对照组IL-18与IFN-γ无显著相关性(P>0.05)。脑出血组IL-18、IFN-γ与LPO、MDA具有正相关性(P<0.05)。结论急性自发性脑出血患者IL-18、IFN-γ均有显著增高,且与氧化应激明显相关。提示IL-18、IFN-γ参与了急性自发性脑出血发生发展的炎性反应过程,急性发病后的氧化应激效应是炎症反应的促发因素。
Objective To investigate the changes of interleukin（IL）-18 and interferon（IFN）-γ in plasma of the patients with acute spontaneuse cerebral hemorrhage,and to observe the role of IL-18 and IFN-γ in the pathogenesis of acute spontaneuse cerebral hemorrhage. Methods Enzyme-linked immunosorbent assay （ELISA） was used to detect the concentration of IL-18,IFN-γ in plas ma of the patients with acute spontaneuse cerebral hemorrhage. Serum level of MDA and LPO was measured by biochemistry. Results MDA and I.PO in the patients with acute spontaneuse cerebral hemorrhage were significantly higher than those in the control group（P〈0. 001）. IL- 18 and IFN-γ in the patients with acute spontaneuse cerebral hemorrhage were significantly higher than those in the control group（P〈0. 001）. Plasma IL-18 and IFN-γ was significantly positively correlated in the patients. IL-18 and IFN-γ in the patients showed positive correlation with MDA and LPO. Conclution The concentration of IL-18 and IFN-γ in the patients with acute spontaneuse cerebral hemorrhage is significantly higher and associated with oxidative stress. This suggests that IL-18, IFN-γ may be both involved in the inflammation process of development of acute spontanouse cerebral hemorrhage.
出处
《重庆医学》
CAS
CSCD
2008年第24期2793-2796,2799,共5页
Chongqing medicine
