摘要
通过建立人参皂苷Rb1与其他8种皂苷间的紫外相对校正因子(RCF),实现只用一个对照品测定人参和三七药材中多个人参皂苷类成分的含量,以解决人参等药材质量控制中,对照品供应不足问题。结果表明,在一定的线性范围内,人参皂苷Rb1与Rg1、Re、R f、Rh1、R c、Rb2、Rb3、Rd间的RCF值分别为1.400,1.215,1.517,1.801,0.944,1.012,1.143,1.135,且在不同实验条件下重现性良好(RSD=0.30%～3.9%)。本方法只需测定人参和三七药材中Rb1的含量,其余人参皂苷含量由其RCF值计算得到,实现一测多评;并与常规外标法比较,两种药材中一测多评法与外标法所得结果均无显著性差异;所建立的校正因子可同时用于人参及三七药材及其相关产品的定量分析及质量评价。
Current quality control patterns are limited to industrial application, for most of the natural chemical reference substances are expensive and unavailable. Herein, a method, quantitative analysis of multi-components with single marker （QAMS）, was established and validated to simultaneously determine nine ginsenosides （ginsenoside Rg1, Re, Rf, Rh1, Rb1, Rc, Rb2, Rb3, Rd） in P. ginseng and four ginsenosides （ginsenoside Rg1, Rh1, Rb1, Rd） in P. notoginseng. Using ginsenoside Rb1 as the contrast, the relative correction factors （RCF） of the other eight ginsenosides were determined by HPLC-DAD. Within the linear ranges, the values of RCF of ginsenoside RbI to ginsenoside Rg1, Re, Rf, Rh1, Rc, Rb2, Rb3 and Rd were 1. 400, 1. 215, 1. 517, 1. 801, 0. 944, 1. 012, 1. 143, and 1. 135, respectively. The RCF had a good reproducibility in various instruments, chromatographic columns （ RSD = 0. 30% - 3.9% ）. According to their RCF, we simultaneously determined nine ginsenosides in P. ginseng only using one marker. In addition, the RCF of ginsenosides were used to simultaneously quantitative analysis of four ginsenosides in P. notoginseng. The results of QAMS method were validated by comparing with that of external standard method, and no obvious significant difference was found.
出处
《药学学报》
CAS
CSCD
北大核心
2008年第12期1211-1216,共6页
Acta Pharmaceutica Sinica
基金
2007年中医药行业科技专项资助项目(200707009)
“十一五”科技支撑计划资助项目(2006BAI08B03-09)
中国中医科学院基本科研业务费自主选题资助项目(2006)
关键词
一测多评
相对校正因子
HPLC
人参皂苷
人参
三七
quantitative analysis of multi-components by single marker
relative correction factor
HPLC
ginsenosides
Panax ginseng
Panax notoginseng
作者简介
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