摘要
目的:建立同时测定三七中多种皂苷成分的RP-HPLC方法。方法:Zorbax Eclipse XDB-C_(18)色谱柱(250mm×4.6 mm,5μm);乙腈-水二元梯度洗脱,乙腈:0～30min,18%;30～35min,18%→30%;35～55min,30%→35%;55～65min,35%→55%;65～70min,55%。流速1.5mL·min^(-1);DAD监测器,检测波长203nm;柱温为40℃。结果:三七皂苷R_1,人参皂苷Rg_1、Re、Rf、Rb_1、Rg_2、Rh_1、Rc、Rb_2、Rb_3和Rd的线性范围分别为0.24～4.75μg(r=0.9999),0.57～11.40μg(r=1.0000),0.50～9.90μg(r=1.0000),0.24～4.80μg(r=1.0000),0.54～10.65μg(r=1.0000),0.18～3.60μg(r=1.0000),0.16～3.10μg(r=1.0000),0.26～5.10μg(r=1.0000),0.25～4.90μg(r=1.0000),0.25～4.95μg(r=1.0000),0.32～6.30μg(r=1.0000);回收率为97.3%～102.8%。结论:RP-HPLC法可同时测定三七中11种皂苷的含量,有利于其质量控制。
Objective：To establish a RP - HPLC method for simultaneous determination of multiple components of saponins of Panax notoginseng. Methods：Zorbax Eclipse XDB -C18 column （250 mm × 4.6 mm ,5μm）with gradient elution was adopted. Solvent A was acetonitrile and solvent B was water. The gradient program of solvent A was as follows：0 -30 min,18% ;30-35 min,18% -30% ;35 -55 min,30% -35% ;55 -65 min,35% -55% ;65 - 70 min,55%. The flow rate was 1.5 mL. min-1. Diode array detector was set at 203 nm and column temperature was 40℃. Results： The linear ranges of notoginsenoside R1, ginsenoside Rg1, Re, Rf, Rb1 , Rg2, Rh1, Re, Rb2, Rb3 and Rd were 0.24 - 4.75 μg （ r = 0. 9999 ） ,0.57 - 11.40 μg （ r = 1. 0000） ,0.50 - 9.90 μg （ r = 1. 0000） ,0.24 - 4.80μg（ r = 1. 0000 ） , 0.54 - 10.65 μg （ r = 1. 0000） , 0.18 - 3.60μg （ r = 1. 0000 ） , 0.16 - 3.10 μg （ r = 1. 0000 ） ,0.26 - 5.10μg （ r = 1. 0000 ） ,0.25 - 4.90μg （ r = 1. 0000 ） , 0.25 - 4.95 μg （ r = 1. 0000 ） , 0.32 -6.30 μg（r = 1. 0000）, respectively. The recoveries of 11 compounds of saponins were within 97. 3% - 102. 8%. Conclusion： 11 components of saponins of Panax nototgirtseng could be simultaneously determined with the developed RP- HPLC method,which will improve the quality control.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第9期1281-1283,共3页
Chinese Journal of Pharmaceutical Analysis
作者简介
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