摘要
目的：测定不同产地决明子中蒽醌类成分的含量。方法：以Inertsil ODS-3为色谱柱，乙腈-0．1％磷酸水溶液为流动相，梯度流速0．8mL·min^-1，检测波长278nm，测定决明子药材中7个蒽醌类化学成分的含量。结果：各类成分的回收率均在95％～105％，含量因产地不同差异较大。结论：HPLC梯度洗脱法测定决明子药材中蒽醌类成分，方法简便、准确，为决明子质量评价和标准制定提供方法。
Objective： To determine the content of 7 anthraquinones in Semen Cassiae. Method： A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0. 1% H3 PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0. 8 mL · min^-1. Result： Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different. Conclusion： The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第23期2797-2799,共3页
China Journal of Chinese Materia Medica
关键词
决明子
HPLC
蒽醌
钝叶素
橙黄决明素
Semen Cassiae
HPLC
anthraquinone
obtusifolin
aurantio-obtusin
作者简介
通讯作者：杨大坚，Tel：（023）89029025，E-mail：yangdaji@hotmail．com
