摘要
目的研究白细胞介素6、8、10(IL-6、IL-8、IL-10)在急性肺损伤中的作用。方法雄性SD大鼠31只,随机分为对照组(n=10)和油酸(OA)组(n=21)(鼠尾静脉注射油酸0.1ml/kg复制急性肺损伤模型),OA组分为2、4、6h3个观察点,每个点7只。对照组鼠尾静脉注射生理盐水0.1ml/kg,观察6h。测定动脉血氧分压(PaO2)、肺湿/干重比、肺泡灌洗液中中性粒细胞(PMN)百分比,光镜观察肺组织形态学变化,半定量肺损伤评分(IQA),用双抗体夹心ELISA法检测血浆、肺组织匀浆IL-6、IL-8和IL-10的含量。结果油酸鼠尾静脉注射可引起肺组织明显的形态学改变。OA组与对照组比较,可见PaO2下降,肺湿/干重比、PMN%、IQA增加(均P<0.01),血浆、肺组织匀浆中IL-6、IL-8、IL-10含量均显著增加(均P<0.01)。血浆、肺组织匀浆IL-6和IL-8水平与急性肺损伤IQA评分呈明显正相关。结论急性肺损伤时炎症因子和抗炎因子均明显升高,IL-6在急性肺损伤时起炎症因子的作用。
Objective To investigate the role of interleukins IL-6, IL-8, and IL-10 in acute lung injury （ALI）. Methods Thirty-one male SD rats were randomly divided into 2 groups： oleic acid （OA） group （n=21） undergoing injection of OA via caudal vein so as to establish rat ALI models, and control group （n=10） undergoing injection of normal saline （NS）. 2, 4, and 6 hours after the OA injection blood samples were collected from the abdominal aorta of 7 rats respectively to undergo blood gas examination. Then the rats were killed with their lungs taken out to undergo microscopy. The rats in the control group underwent blood sample collection and than killed 6 h after the NS injection. Index of quantitative assessment （IQA） was calculated. Double-antigen ssandwich ELISA was used to detect the IL-6, IL-8, and IL-10 levels in the plasma and lung tissue homogenate. The partial pressure of oxygen （PaO2） was measured. The lung tissue wet/dry （W/D） ratio was calculated. Bronchoalveolar lavage fluid was obtained from the left lung and the percentage of polymorphonuclear leukocytes （PMN%） was tested. Results Obvious inflammatory changes were seen in the lungs of the OA group. The PaO2 level 6h after the injection of the OA group was （65.21 ±7.81） mmHg, significantly lower than that of the control group [（99.94 ±8.85） mmHg, P〈0.01]. The W/D ratio 6 h after injection of OA group was （5.81 ±0.434）, significantly higher than that of the control group [（19 ±0.50）, P 〈0.01]. The PMN% 6h after injection of the OA group was （87.33 ±2.73）%, significantly higher than that of the control group [（3.14 ±1.57）%, P〈0.01]. The IQA score 6h after injection of the OA group was （6.67 ±0.82）, significantly higher than that of the control group [（0.50 ±0.55）, P 〈 0.01]. The concentrations of IL-6, IL-8, and IL-10 in plasma and lung tissue homogenate of the OA group were all significantly higher than those of the control group （all P 〈0.01）. Pearson＇s 2-tailed test showed that IQA score was positively correlated with the levels of IL-6 and IL-8 in lung tissue homogenate and plasma （all P〈0.05）. Conclusion The concentrations of inflammatory factors and anti-inflammatory factor all increase significantly in ALI. IL-6 plays a role of inflammatory factor in the process of ALI.
出处
《中国急救复苏与灾害医学杂志》
2008年第12期728-730,733,共4页
China Journal of Emergency Resuscitation and Disaster Medicine
基金
国家重点基础研究发展规划项目(2006CB503807)
国家自然科学基金资助项目(30425010
30571971
30630031)
关键词
急性肺损伤
白细胞介素
中性粒细胞
Acute lung injury
Interleukin
Polymorphonuclear leukocytes
作者简介
关岚，硕士，医师
通讯作者：赵斌，E—mail：zhaohin60@yahoo．com．cn，电话：010—58516789
