摘要
目的观察二至天癸颗粒含药血清对初老小鼠卵巢颗粒细胞体外分泌功能的影响。方法采用血清药理学方法,以不同含药量的初老小鼠血清,与体外培养的颗粒细胞共同培养48h,观察二至天癸颗粒含药血清对初老小鼠卵巢颗粒细胞雌激素(E2)、孕酮(P)分泌量(化学发光免疫测定法)以及抑制素B(INHB)分泌量(ELISA法)的影响。结果二至天癸颗粒含药血清可明显增加颗粒细胞E2、P、INHB的分泌量。结论二至天癸颗粒可通过调节颗粒细胞的分泌功能进而改善初老小鼠卵巢功能。
Objective To study the effect of serum contained Erzhi Tiangui Granule on the secretion function of ovarian granulosa cells from primary senile mice. Methods The serum with decoction from primary senile mice was added to cultured granulosa cells and incubated for 48 h. Then the concentration of estradiol （E2）, progesterone （P） and inhibin B （INHB） in cultured medium were measured by chemiluminescence immunoassay （CLIA） and Sandwich ELISA respectively. Results The serum contained Erzhi Tiangui Granule could increase the secretion of E2, P and INHB. Conclusion Erzhi Tiangul Granule can improve the ovarian function of primary senile mice by regulating the secretion function of granulosa ceils.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第12期47-48,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(90209058)
关键词
二至天癸颗粒
卵巢颗粒细胞
雌激素
孕酮
抑制素B
含药血清
Erzhi Tiangui Granule
ovarian granulosa cell
estradiol
progesterone
inhibin B
serum contained drug
作者简介
通讯作者：连方，Tel：0531—82611558，E-mail：f_lian@163．com
