摘要
目的:观察正常胃黏膜、肠上皮化生、异型增生和早期胃癌中抑癌基因PTEN的蛋白表达,探讨PTEN表达在胃癌早期发生过程中的作用.方法:共收集57例病变,按病理级别分为慢性胃炎伴肠上皮化生14例,异型增生27例,早期胃癌16例,另取20例正常胃黏膜作为对照.采用EnVision免疫组化方法检测各组黏膜中PTEN蛋白表达情况,并分析其与临床病理特征的关系.结果:PTEN在正常胃黏膜和肠上皮化生中全部表达,在异型增生和早期胃癌中的阳性率分别为92.6%(25/27),75.0%(12/16).PTEN在正常胃黏膜、肠上皮化生和异型增生三组间的表达无显著差异,在早期胃癌中的表达较前三组明显降低(P<0.05).PTEN表达强度与病理级别呈明显的负相关(r=-0.488,P<0.01).PTEN蛋白表达与患者年龄、性别、病变部位及Hpylori感染无关(P>0.05),但与血清CEA水平显著相关(P=0.001).结论:作为一种具有磷酸酶活性的抑癌基因,PTEN蛋白低表达在胃癌早期发生过程中发挥着重要的作用.
AIM： To investigate the correlation of phosphatase and tensin homolog deleted on chromosome ten （PTEN） protein expression and clinicopathological features in the precancerous lesions and early gastric cancer, and to explore its clinical implication in early development of gastric cancer.
METHODS： A series of 20 normal gastric mucosal samples, 14 intestinal metaplasia （IM）, 27 dysplasia and 16 early gastric carcinomas were examined for the expression of PTEN using En-Vision immunohistochemistry. Correlation between PTEN expression and clinicopathological features was analyzed.
RESULTS： The positive rates of PTEN protein were 100% （20/20）, 100% （14/14）, 92.6% （25/27） and 75.0% （12/16） in normal gastric mucosa, IM, dysplasia and early gastric carcinoma, respectively. The positive rate of PTEN protein expression was significantly lower in early gastric carcinoma than the other three groups （P 〈 0.05）. PTEN protein expression level was negatively correlated with the pathological severity（r =-0.488, P 〈 0.01）. The expression of PTEN was markedly associated with the serum CEA level （P = 0.001）, but not with age, sex, H pylori infection or with location of lesions （P 〉 0.05）.
CONCLUSION： PTEN, a tumor suppressor gene with phosphatase activity, plays an important role in the early carcinogenesis of gastric carcinoma owe to its reduced expression.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第32期3626-3630,共5页
World Chinese Journal of Digestology
基金
江苏省卫生厅科教兴卫工程重点人才资助项目
N o.RC2007003~~
关键词
PTEN蛋白
早期胃癌
癌前病变
Phosphatase and tensin homolog deleted on chromosome ten protein
Early gastric cancer
Precancerous lesions
作者简介
张斌，南京大学医学院在读硕士生，主要从事消化系肿瘤的表观遗传研究．
通讯作者：邹晓平，210008，江苏省南京市中央路321号．南京大学医学院附属鼓楼医院消化科．zouxiaoping795@hotmail．com电话：025-83106666-20602
