摘要
目的:考察硫酸铵梯度法制备马钱子碱脂质体的影响因素。方法:采用Sephadex G-50凝胶柱分离测定包封率,考察各工艺及处方因素对包封率的影响。结果:马钱子碱脂质体的包封率与硫酸铵浓度、乙醇体积、磷脂浓度等因素存在显著的正相关性。结论:硫酸铵梯度法可以用于马钱子碱脂质体制备并得到满意的包封率。硫酸铵浓度、乙醇体积和磷脂浓度是影响包封率的重要因素。
Objective： To study the influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients. Methods： The brucine liposomes were separated by Sephadex G-50, and the influence of various factors on the entrapment effieieneies were investigated. Results： The entrapment efficiency was enhanced by increased ammonium sulfate concentration, ethanol volume and PC concentration. Conclusion： Burcine liposomes prepared by ammonium sulfate transmembranee gradients can get a high entrapment efficiency, the main influencing factors were ammonium sulfate concentration, ethanol volume and PC concentration.
出处
《中药材》
CAS
CSCD
北大核心
2008年第9期1410-1412,共3页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助项目(30701111)
关键词
硫酸铵梯度法
马钱子碱
脂质体
Ammonium sulfate transmembrane gradients
Brueine
Liposome
作者简介
通讯作者：陈军，E-mail：chenjun75@163．com。
