摘要
采用1:2的氯仿:甲醇超声提取鸭跖草成分,并用石油醚、乙酸乙酯和正丁醇依次进行萃取,利用DPPH法、α-脱氧核糖法和铁氰化钾还原法进行抗氧化实验。同时与BHT和没食子酸(GA)2种合成抗氧化剂进行对照。结果显示乙酸乙酯提取物(EAF)具有较高的抗氧化活性。对其进行硅胶柱层析分离,得到6种纯化的组分(EAF1～EAF6),其中EAF4具有最高的抗氧化活性,在实验所测4个浓度下,清除DPPH自由基能力均比BHT强,在10μg/ml和50μg/ml浓度时,比GA强。其清除羟自由基能力在所有测定浓度下均比2种人工合成抗氧化剂强。其还原能力在所有测定浓度下均比BHT强,但比GA弱。
Commelina communis L. （CCL） was extracted with methanol-chloroform （2：1）, and then the extract was partitioned with petroleum ether, EtOAc and BuOH successively. Antioxidant activities （AA） of different end extracts were evaluated by three different methods, namely, 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical scavenging assay, deoxyribose assay and potassium ferricyanide reduction method, and compared with those ofbutylated hydroxytoluene （BHT） and gallic acid （GA）. The results showed that the ethyl acetate-soluble fraction exhibits the highest AA at four concentration levels （0.4, 2, 10 and 50 μg/ml）. It can be further fractionated into six subfractions EAF1-EAF6 by silica gel vacuum liquid chromatography. Among them EAF4 is found to be the most effective subfraction by the three assays, which has stronger AA than BHT at the four concentration levels and greater AA than GA at the concentration of 10μg/ml and 50μg/ml by the DPPH assay. While, by the deoxyribose assay the EAF4 exhibits higher AA than BHT and GA at all of the four concentration levels and by the potassium ferricyanide reduction methods it exhibits higher AA than BHT and lower AA than GA.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第9期55-58,共4页
Food Science
关键词
天然抗氧化剂
鸭跖草
DPPH
羟自由基
还原能力
natural antioxidant
Commelina communis L.
DPPH
hydroxyl radical
reducing power
作者简介
黄海兰（1963-），女，教授，硕士，主要从事天然产物化学研究。E-mail：qdhuang07@163．com
