摘要
选用姜块进行3步复合消毒法脱菌试验,试验结果表明,试验最佳处理组合为0min5%次氯酸钠(NaClO)、0.5min70%乙醇(C2H5OH)和15min0.1%升汞(HgCl2)。
Explant of ginger has high polluting rate in tissue culture, which is the keystone for the tissue culture. Multiple factor experiments for stem apex of ginger were made in order to reduce the polluting rate, to increase induction rate of adventitious bud and survival ratio of stem apex of ginger. Three steps of complex sterilization in the experiment were used, the results showed that the combination of 0 min 5% NaClO, 0.5 rain 70% C2H5OH and 15 min 0.1% HgCl2 was the best.
出处
《长江蔬菜》
北大核心
2008年第09X期60-62,共3页
Journal of Changjiang Vegetables
关键词
生姜
组织培养
脱菌
Ginger
Tissue culture
Sterilization
作者简介
薛寒青（1965-），女，硕士，副研究员，研究方向为蔬菜和花卉的组织培养和脱毒，电话：0971—5312581。E-mail：hanqx77@sina．com
