摘要
以物质的太赫兹吸收全谱为特征指纹可以进行混合物的成分分析。生化物质容易受外界影响而变性,使用光子能量只有毫电子伏特的太赫兹波来分析物质成分不会带来有害的电离辐射。文章使用太赫兹谱成分分析法对腺苷、胸苷、鸟苷、胞苷、尿苷的混合物做了分析,实验结果表明该方法可以较为准确地分析出这几种核苷混合物的成分及其含量,相对误差在10%以内,并对误差产生的原因做了简要分析。该项研究表明,此方法简单有效而且对样品无损。有可能成为生化物质成分分析的一种新手段,从而进一步拓展太赫兹谱的应用领域。
Adenosine, thymidine, guanosine, cytidine and uridine form the building blocks of ribose nucleic acid （RNA） and deoxyribose nucleic acid （DNA）. Nucleosides and their derivants are all have biological activities. Some of them can be used as medicine directly or as materials to synthesize other medicines. It is meaningful to detect the component and content in nucleosides mixtures. In the present paper, components and contents of the mixtures of adenosine, thymidine, guanosine, cytidine and uridine were analyzed. THz absorption spectra of pure nucleosides were set as standard spectra. The mixture’s absorption spectra were analyzed by linear regression with non-negative constraint to identify the components and their relative content in the mixtures. The experimental and analyzing results show that it is simple and effective to get the components and their relative percentage in the mixtures by terahertz time domain spectroscopy with a relative error less than 10%. Component which is absent could be excluded exactly by this method, and the error sources were also analyzed. All the experiments and analysis confirms that this method is of no damage or contamination to the sample. This means that it will be a simple, effective and new method in biochemical materials analysis, which extends the application field of THz-TDS.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2008年第9期1990-1993,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(10574134)
中国科学院知识创新工程项目资助
关键词
太赫兹谱学
定量分析
核苷
Terahertz spectroscopy
Quantitative analysis
Nucleotide
作者简介
张增艳，1980年生，中国科学院上海应用物理研究所在读博士
通讯联系人 e-mail：tqxiao@sinap．cn
