摘要
目的构建中性粒细胞减少合并侵袭性肺曲霉病(IPA)的动物模型。方法40只小鼠随机分为4组:A组为模型组,环磷酰胺150mg/kg于接种前4d及接种前1d腹腔注射,接种当天给予1%戊巴比妥45mg/kg腹腔注射麻醉后,1×108个/mL烟曲霉孢子悬液40μL滴鼻。B组为非免疫抑制接种对照组,除以生理盐水代替环磷酰胺外,其余操作同模型组。C组为免疫抑制对照组,除以生理盐水代替烟曲霉孢子悬液滴鼻外,其余操作同模型组。D组为空白对照组,以生理盐水代替环磷酰胺腹腔注射并以生理盐水代替烟曲霉孢子滴鼻,其余操作同模型组。模型建立成功的判断标准:肺病理切片见坏死灶、肺泡内出血,PAS染色见45°角分叉的分隔菌丝或孢子;组织培养烟曲霉阳性。结果肺大体观察、病理切片H-E染色及PAS染色和肺组织培养结果表明,A组10只小鼠在接种烟曲霉3d后均发生了IPA,B、C、D组小鼠均无IPA发生。结论本法可成功构建中性粒细胞减少小鼠IPA模型。
Objective To construct a model of invasive pulmonary aspergillosis （IPA） in neutropenic mouse. Methods Forty mice were randomly assigned into 4 groups： group A （model group） received cyclophosphamide （intraperitoneal; 150 mg/kg, Day-4, Day-1） and Aspergillus fumigatus conidia suspension （intranasal, 40 /μL/mouse,1×10^8/mL, Day 0）; group B received normal saline instead of cyclophosphamide, and the other procedure is the same as group A; group C received normal sa- line instead of A. fumigatus conidia suspension, and other procedure is the same as group A~ group D received only normal saline. Pathologic examination and culture of the lungs of mice were evaluated to determine whether the IPA model was successfully constructed. Results IPA was confirmed in all the 10 mice of group 3 days after inoculation of A. fumigatus conidia based on pathologic examination and culture of lung tissues. No IPA was found in any mouse of group B, C and D. Conclusions The IPA model in neutropenic mouse is constructed successfully.
出处
《中国感染与化疗杂志》
CAS
2008年第5期373-376,共4页
Chinese Journal of Infection and Chemotherapy
基金
上海市卫生局科技发展基金项目(044019)
作者简介
荣令（1975-），男，主治医师，在读博士，主要从事肺部真菌感染研究。
通讯作者：周新，E—mail：xzhou53@163.com。
