摘要
在临床应用中,低功率He-Ne激光(632.8 nm)能促进骨骼肌修复,加速创伤愈合,降低牙齿的超敏感性,减缓疼痛等。大量研究表明:低功率He-Ne激光能调节细胞的众多行为,如细胞增殖、分泌、迁移、粘附、蛋白质合成和基因表达等。但低功率He-Ne激光调节细胞行为的分子机制并未阐明,考察低功率He-Ne激光照射后细胞内活性氧自由基的产生水平和游离Ca2+浓度是否会发生变化,通过激光扫描共聚焦显微镜,分别利用H2DCFDA和Fluo-3/AM这两种荧光探针,检测到经He-Ne激光照射后,肺腺癌细胞内活性氧自由基的水平上调以及游离Ca2+浓度增加。该研究为低功率He-Ne激光的生物光刺激效应提供了可能的分子机理。
Low power He-Ne laser （632.8 nm） irradiation have been applied clinically to promote skeletal muscle regeneration, accelerate wound healing, decrease dental hypersensitivity, relieve pain, and so on. Many researches indicate that low power He-Ne laser irradiation could modulate various biological processes such as promoting cell proliferation, secretion, migration, adhesion, protein synthesis, gene expression. However, the mechanism of photo-stimulative effect has not been fully clarified. In this study, we investigated whether low power He-Ne laser irradiation induced the generation of ROS and the increase of Ca^2＋. Using two fluorescence probes H2DCFDA and Fluo-3/AM, we found that the generation of ROS and the release of Ca^2＋ were induced in the human lung adenocarcinoma cells （ ASTC-a-1 ） by 0.8 J/cm^2 He-Ne laser irradiation observed with the laser scanning confocal microscope （LSM）. Our study provided a possible molecular mechanism for the photo-biostimulative effect of low power He-Ne laser irradiation.
出处
《激光生物学报》
CAS
CSCD
2008年第4期427-431,共5页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(30470494
30627003)
广东省自然科学基金项目(7117865)
作者简介
张军涛（1981-），女，研究生，主要研究激光在生命科学研究中的应用。（电话）020-85211436-8207；（电子信箱）zhangjt@scnu．edu．cn
通讯作者：（电话）020-85210089；（传真）020-85216052；（电子信箱）xingda@scnu．edu．cn
