摘要
采用微波辅助提取金银花中的绿原酸。考察了溶剂种类及浓度、提取时间、液固比、溶剂pH、提取次数等对绿原酸得率的影响;结合正交实验设计确定了绿原酸的最优提取工艺条件:液固比15,φ=0.4的乙醇溶液提取2次,每次60 s。将优化后的微波提取结果与其他方法比较,结果表明:微波法具有操作简单、快速高效、节能环保等优点。
Chlorogenic acid was extracted from flos lonicerae by microwave-assisted extraction（MAE）. Ultraviolet（UV） ray was used for the quantitative and qualitative determination of chlorogenic acid from flos lonicerae. The effects of solvent types and concentration, MAE time, liquid/solid ratio, pH of solvent and extraction degrees on extraction of chlorogenie acid were discussed. Orthogonal experiment was employed to investigate the optimum technological condition. The optimum extraction conditions were as follows： ratio of enthanol solution （φ=0.4） to solid, 15 ; MAE time, 1 min; extraction twice. Compared with other techniques, MAE is fast, simple, high efficient, energy-saving and environmental friendly.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第4期543-546,552,共5页
Journal of East China University of Science and Technology
基金
国家自然科学基金资助项目(20006003)
关键词
微波辅助提取法
金银花
绿原酸
microwave-assisted extraction
flos lonicerae
chlorogenic acid
作者简介
刘志平（1983-），男，河南安阳人，硕士研究生，研究方向：传质与分离工程。
通讯联系人：韩伟，Email：whan@ecust．edu．cn
