摘要
采用超声波浸提法从普洱茶中提取多糖,用硫酸-苯酚法显色,在490nm处运用分光光度计测其含量;考察了超声波功率,浸提固液比,超声波处理时间对多糖提取率的影响,并以正交试验设计优化工艺条件,结果表明:影响普洱茶多糖提取率因素的主次关系是功率>浸提固液比>时间。正交实验最佳条件:功率800W,固液比1∶50,时间25min,为提取普洱茶多糖的开发提供了可靠的实验依据。
Ultrasonic-assisted extraction is adopted to extract polysaccharide from Pu'er Tea, using sulphuric acid-phenol method and the content of polysaccharide is determined by the spectrophotometer at 490 nm. The effects of Ultrasonic intensity, ratio of solid and liquid, Uhrasonication time, diameter of material on extraction efficiency was studied.h was found through the orthogonal experiments that the optimum technological conditions was Ultrasonic wave intensity: 800W, time:25min, ratio of material and solove: 1:50,which provide an important ethod for industrial extraction of polysaccharide of Pu'er Tea.
出处
《食品研究与开发》
CAS
北大核心
2008年第4期119-122,共4页
Food Research and Development
基金
国家自然科学基金
广东省人民政府自然科学联合基金(U0632010)
关键词
超声
提取
普洱茶
多糖
ultrasonic wave
extraction
Pu'er Tea
polysaccharide
作者简介
安卫征(1980-),男(汉),在读硕士,专业方向:中药药理学。