摘要
【目的】探讨白皮松体细胞胚胎发生过程中各项生理生化指标与体细胞胚胎发生、发育的关系。【方法】以白皮松体细胞胚发生诱导阶段不同发育时期的胚性愈伤组织(EC)和非胚性愈伤组织(N-EC)为材料,对其不同培养时间(5,15,25和35 d)的可溶性蛋白质、可溶性糖和淀粉含量及抗氧化物酶(POD,SOD,CAT)活性进行研究。【结果】EC中可溶性蛋白质和可溶性糖含量均在培养25 d时达到峰值,N-EC中可溶性蛋白和可溶性糖含量在培养15 d时达到峰值;EC中淀粉含量呈现先上升后下降再上升的变化趋势,N-EC淀粉含量一直呈下降趋势。EC中抗氧化物酶活性明显高于N-EC,SOD活性在培养15 d时达到峰值,表明SOD在体细胞胚诱导初期起主导作用;EC的CAT和POD活性均在培养25 d时达到峰值,说明CAT和POD在体细胞胚发生过程中对胚性细胞的进一步分化和发育起主导作用。【结论】白皮松体细胞胚胎发生诱导阶段,EC的生理生化代谢活性高于N-EC。
[Objective] The study investigated the correlation between physiological and biochemical characteristics and somatic embryogenesis in Pinus bungeana. [Method] Different developmental stages of Callus were induced from immature zygotic embryos. The contents of soluble protein, soluble sugar and starch,the different activities of antioxidant enzymes between embryogenic callus （EC） and non-embryogenie callus （N-EC） were examined. [Result] The soluble protein and soluble sugar content of EC reached maximum on 25th d,and those of N-EC reached maximum on 15^th d. The starch content of EC presented to rise first and then drop,and reached its maximum on 15^th d. while N-EC presented the drop tendency. All antioxides enzyme activities of EC were much higher than those of N-EC. The SOD activity peak value achieved on 15^th d of EC,so SOD played a dominant role in the induction of somatic embryos. The peak value achieved on 25th d for CAT and POD,playing an dominating role in the differentiation and development of somatic embryos. [Conclusion] Physiological biochemistry metabolic activity of EC is much higher than NEC during the process of inducing somatic embryos in Pinus bungeana.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2008年第8期151-155,160,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
宁夏大学青年教师科研启动项目(QNZ0618)
陕西省科技发展计划项目(2003K02-G8-03)
关键词
白皮松
体细胞胚胎发生
胚性愈伤组织
非胚性愈伤组织
生理生化特性
Pinus bungeana
embryogenic callus
ono-embryogenic callus
Somatic embryogenesis
Physiological and biochemical characteristic
作者简介
李茜（1981-），女，陕西汉中人，助理研究员，硕士，主要从事林木遗传育种研究。E-mail：li_q@nxu．edu．cn
