摘要
以诱导产生的栓皮栎不同基因型的胚性愈伤组织和非胚性愈伤组织为材料,对其过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及同工酶谱型进行了研究。结果表明:胚性愈伤组织中抗氧化物酶活性均明显高于非胚性愈伤组织。不同基因型的胚性愈伤组织或非胚性愈伤组织的抗氧化物酶活性之间差异不显著,但同一基因型的胚性和非胚性愈伤组织之间均存在极显著差异。不同抗氧化物酶同工酶谱具有特异性,且显示与酶活性一致的变化趋势。
Callus were induced from three different genotypes of immature zygotic embryos in Quercus variabilis. The activities and isozyme phenotypes of catalase （CAT）,peroxidase （POD） and superoxide dismutase （SOD） of embryogenic callus and non-embryogenic callus were examined. The results showed that all antioxides enzyme activities of embryogenic callus were clearly higher than those of non-embryogenic callus. Differences of the activities of embryognic callus or non-embryogenic callus amony different genotypes were not significant. However, contrary appeared in activities of same genotype in embryogenic callus and non-embryogenic callus. Different antioxides had special isozype phenotypes. The patterns were corresponding with their activities.
出处
《西北林学院学报》
CSCD
北大核心
2007年第3期67-70,共4页
Journal of Northwest Forestry University
基金
国家林业局"948"项目(2006-4-54)
西北农林科技大学植物遗传育种专项(05YZ039)
作者简介
辛福梅（1981-），女，甘肃武威人，在读硕士，主要从事林木遗传育种和林木生物技术研究工作。
通讯作者：张存旭。
