摘要
目的测定香青兰全草中总三萜酸成分含量。方法以齐墩果酸为指标,采用香草醛-冰醋酸及高氯酸显色后用分光光度法测其含量。结果齐墩果酸的线性范围是8.48-42.4μg·ml^-1,测得香青兰中总三萜酸含量约为14.9 mg/g,RSD为1.23%。结论用大孔吸附树脂对香青兰除杂后富集利用显色法测定总三萜酸是可行的,该方法具有简便、快速、准确、重复性好等优点。
Objective To determine the content of the Tritepenoidic Acid in Dracocephalum moldavica L . Methods With oleanolic acid as the standard,5% Vanillin- Acetic acid and perchloric as the developer,colorimetric method was applied. Results A good linearity was found in the concentration range of 8.48 - 42.4 μg · ml^-1 for oleanolic acid, the average recovery was 99.1% with RSD 1.23%. The average content of Dracocephalum moldavica L. was 14.9 mg · g^-1 Conclusion Macroreticular resin - Colorimetric method is suitable for the determination of total tritepenoidic acid from Dracocephalum moldavica L. This method is simple, the result is stable, and has good repeatability.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第5期1180-1181,共2页
Lishizhen Medicine and Materia Medica Research
关键词
香青兰
三萜酸
大孔吸附树脂
比色法
齐墩果酸
Dracocephalum moldavica L
Tritepenoidic Acid
Macroreticular resin
Colorimetric method
Oleanolic acid
作者简介
田树革（1968-），男（汉族），山东梁山人，现任新疆医科大学中医学院副教授，学士学位，主要从事中药化学和中药制剂分析教学及科研工作．
