摘要
目的:通过小鼠体内外实验观察肿瘤细胞是否可以诱导CD4+CD25+Treg的分化增殖。方法:将小鼠的红白血病瘤细胞系FBL3接种于C57BL/6小鼠腹壁皮下,流式细胞仪检测小鼠外周血、脾脏及瘤组织CD4+CD25+Treg细胞含量,RT-PCR检测小鼠脾脏及瘤组织Foxp3 mRNA的表达;体外实验检测FBL3细胞培养上清液对小鼠脾脏CD4+CD25+Treg细胞的作用。结果:荷瘤鼠外周血CD4+CD25+Treg比例与正常鼠比较无统计学差异(P>0.05),但荷瘤鼠脾脏CD4+CD25+Treg比例显著高于正常对照(P<0.01),荷瘤小鼠脾脏组织Foxp3 mRNA的表达量明显增加;体外实验表明FBL3培养上清液促使CD4+CD25+Treg细胞比例增高,并诱导Foxp3 mRNA表达增加。结论:FBL3细胞及其分泌的可溶性物质能诱导CD4+CD25+Treg细胞的增殖,说明是肿瘤的发生促进了CD4+CD25+Treg增高。
Objective： To explore if the tumor cells could induce the proliferation of CD4^＋CD25^＋ Treg in mice by in vitro and in vivo. Methods：The erythroleukemic cell line FBL3 cells were inoculated at abdominal cavity. CD4^＋CD25^＋ subset in peripheral blood and spleen lymphocytes was analyzed by flow cytometry;The level of Foxp3 mRNA was tested by RT-PCR; In vitro experiments were used to see if the supernatant medium of cultured leukemia cell line FBL3 could affect the proliferation of CD4^＋CD25^＋ Treg cells.Results：No difference was observed in the proportion of CD4^＋CD25^＋ Treg cells in peripheral blood lymphocytes between tumor-bearing mice and normal mice（ P 〉 0.05）, while the proportion of CD4^＋CD25^＋ Treg cells in spleens of tumor-bearing mice was increased significantly compared with that of normal mice （P 〈 0.01）.The level of Foxp3 mRNA in spleens of tumor-bearing mice was significantly higher than that of the control. It was indicated that the supernatant derived from FBL3 upregulated the proportion of CD4^＋CD25^＋ Treg cells and the level of corresponding Foxp3 mRNA in vitro. Conclnsion：The leukemic cells and their solube factors can induce the increase of CD4^＋CD25^＋ Treg and tumorigenesis may facilitate the pro- liferation of CD4^＋CD25^＋ Treg cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第6期518-520,525,共4页
Chinese Journal of Immunology
基金
山西医科大学博士启动基金资助
作者简介
张帆（1971年-），女，博士，讲师，主要从事肿瘤免疫研究，E-mail：zhangfan11688@126．com；
通讯作者及指导教师：王宏伟（1966年-），男，博士，教授，博士生导师，主要从事白血病分子生物学研究，E-mail：wanghw68@hotmail．com。
