摘要
目的了解氟虫腈原药的毒性。方法将清洁级SD大鼠随机分为氟虫腈高(25mg/kg)、中(10mg/kg)、低剂量组(4mg/kg)和对照组(2%吐温80水溶液),每组20只,雌雄各半。采用经口灌胃方式进行染毒,10ml/kg,每日1次,连续染毒90d。按照GB15670—1995《农药登记毒理学试验方法》中亚慢性毒性试验方法进行检测。结果高剂量组动物出现精神萎靡、活动减少、被毛蓬松等症状;中、低剂量组动物未见明显的中毒症状。各组大鼠体重、血常规(白细胞总数、红细胞总数、血红蛋白含量、血小板计数)、尿常规(尿蛋白、尿糖、酮体、红细胞、白细胞、胆红素、比重、pH值、亚硝酸盐、尿胆原)间比较,差异均无统计学意义(P>0.05)。高、中剂量组雄性大鼠血清中谷草转氨酶(AST)活力高于对照组,差异均有统计学意义(P<0.05)。高、中剂量组大鼠肝脏系数大于对照组,差异均有统计学意义(P<0.05)。病理组织学检查显示,高、中剂量组肝细胞点、灶状坏死;中央静脉周围肝细胞体积增大明显,浊肿,胞浆疏松或变空,少数肝细胞明显变大近气球样变。低剂量组未见明显病理变化。结论氟虫腈原药具有肝毒性,最大无作用剂量为4mg/(kg·d)。
Objective To study the subchronic toxicity of fipronil in rats and to protect and improve the environment and human health. Methods 80 SD rats were randomly divided into four groups, 20 in each groups （10 males and 10 females）. The rats were treated with fipronil by gavage, in doses of 25, 10, 4, 0 mg/kg, once a day, 10 mL/（kg· d）, for 90 consecutive days. The test method was according to GB15670-1995. Results The acute toxicity symptom of rats in high dose showed that cachexia, dull, hair floppy. No acute toxicity symptoms were found in the moderate and low dose groups. No significant difference was found in the serum and urine routine test indexes. Liver index increased significandy in the high dose （25 mg/kg）and moderate dose （10 mg/kg） exposed rats. The activity of aspartateaminotransferase （AST） in the serum increased only in male rats. No significant changes in indicators were found in the low exposed rats. The histopathologic examination showed that in the high and moderate dose groups, in the liver, spot and focus necrosis, the hepatocytes around central veins became bigger and cloudy swelling, some hepatocytes presented ballooning degeneration. No obvious pathological change was seen in the low dose group. Conclusion Fipronil has a toxicity to the liver, based on 90 day oral toxic test in rats, no-observed adverse effect level （NOAEL） is 4 mg/（kg· d）.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第5期417-419,F0003,共4页
Journal of Environment and Health
关键词
农药
氟虫腈
转氨酶类
大鼠
Pesticides
Fipronil
Transaminase
Rats
作者简介
王蕊（1960-），女，研究员，从事卫生毒理学研究。
