摘要
目的：建立鸡肉中7种氟喹诺酮类药物的高效液相色谱测定方法。方法：使用Cloversil C18分析柱（250mm×4．6mm，5μm），采用甲酸水溶液-乙腈体系作为流动相，程序波长荧光检测器检测。结果：方法的线性范围为0．3～100μg／L，相关系数（r）大于0．9989；检出限为0．1～0．3μg／kg，定量限为0．3～1．0μg／kg；7种氟喹诺酮类药物在鸡肉中的平均回收率分别为87．9％～100．2％；相对标准偏差为1．1％～5．2％和1．3％～9．3％。结论：该方法准确灵敏、精密度好，用于鸡肉中氟喹诺酮类药物测定，结果满意。
Objective：To establish a method for simultaneous determination of seven fluoroquinolone drugs （fleroxacin, norfloxacin, ciprofloxacin, enoxacin, levofloxacin, lomfloxacin, enrofloxacin） by HPLC in chicken. Methods： HPLC separation was carried out on a Cloversil Cls column （250 mm × 4.6 mm, 5μm） using formic acid aqueous solution acetoutrihe system as mo- bilephase, and determined by programmable fluorescence detection. Results：Linear range was 0.3 - 100 μg/L with correlation co- efficients more than 0. 9989. The limits of detection was 0. 1 - 0. 3 μg/kg, and the limits of quantification was 0. 3 - 1.0 μg/ kg. The mean recoveries for each analyte in chicken ranged from 87.9% to 100. 2% with relative standard deviation below 5.2% at 1 -100 μg/kg fortification level. Conclusion：The method is precise, accurate and sensitive to determine fluoroquinolone drugs in chicken.
出处
《中国卫生检验杂志》
CAS
2008年第4期595-596,606,共3页
Chinese Journal of Health Laboratory Technology
作者简介
马纪伟（1969-），男，硕士，讲师，主要从事有机化学分析研究。
