摘要
【目的】研究双氢青蒿素对人红白血病K562细胞DNA的损伤作用。【方法】体外培养人红白血病K562细胞,加不同浓度双氢青蒿素处理24 h后,MTT法测定IC50;用单细胞凝胶电泳技术观察双氢青蒿素对K562细胞DNA的损伤情况。【结果】双氢青蒿素处理细胞后,MTT检测IC50为8×10-5mol/L;单细胞凝胶电泳显示双氢青蒿素作用24 h后可见明显的彗星状拖尾,而对照组细胞呈现明亮圆点。【结论】双氢青蒿素可抑制K562细胞的生长,对其DNA有致损效应。
[Objective] To investigate the effect of damage of dihydroartemisinin （DHA） on leukemic K562 cell DNA. [Methods] The DNA damage of K562 ceil induced by DHA was studied in vitro by MTT and single ceil gel electrophoresis. [Results] K562 ceils were treated with DHA of different concentrations, the IC50 value was 8 ×10^-5 mol/L. Single ceil gel electrophoresis showed comet-like tails, but control group showed bright dot. [Conclusions] DHA can inhibit the proliferation and damage of DNA of K562 ceils.
出处
《武警医学院学报》
CAS
2008年第1期4-6,F0003,共4页
Acta Academiae Medicinae CPAPF
基金
天津市科委课题资助项目(05YFJMJC08200)
武警医学院院级科研项目(MY2005-6)
关键词
双氢青蒿素
单细胞凝胶电泳
DNA损伤
Dihydroertemisinin
Single ceil gel electrophoresis
DNA damage
作者简介
谢红（1977-），女，籍贯黑龙江，生物化学与分子生物学硕士，讲师，主要从事肿瘤细胞凋亡及其机制的研究。
